Chromophore-assisted laser inactivation of cellular proteins.

  title={Chromophore-assisted laser inactivation of cellular proteins.},
  author={A E Beermann and Daniel G. Jay},
  journal={Methods in cell biology},
A molecular understanding of biology requires that we establish the in situ functions of the proteins in cellular processes. To address this, we developed chromophore-assisted laser inactivation (CALl) for probing the in vivo function of proteins. CALl inactivates specific proteins in living cells by using non-blocking antibodies conjugated with malachite green (MG) dye. MG absorbs 620 nm laser light (which is not absorbed by cells) to generate short lived free radicals with limited range of… CONTINUE READING

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