Chromogenic substrates for horseshoe crab clotting enzyme. Its application for the assay of bacterial endotoxins.

@article{Iwanaga1978ChromogenicSF,
  title={Chromogenic substrates for horseshoe crab clotting enzyme. Its application for the assay of bacterial endotoxins.},
  author={Sadaaki Iwanaga and Takashi Morita and Toshie Harada and Satoko Nakamura and Makoto Niwa and Katsumi Takada and Tetsuya Kimura and Sachiko Sakakibara},
  journal={Haemostasis},
  year={1978},
  volume={7 2-3},
  pages={
          183-8
        }
}
An endotoxin-activated hemocyte lysate from the horseshoe crab (Tachypleus and Limulus) was found to hydrolyze Bz-Ile-Glu-(gamma-OR)-Gly-Arg-p-nitroanilide (PNA), Bz-Val-Gly-Arg-PNA, Boc-Val-Leu-Gly-Arg-PNA, and Boc-Leu-Gly-Arg-PNA, all of which have the COOH-terminal Gly-Arg sequence. This amidase activity was due to a clottting enzyme contained in the lysate. Furthermore, the amidase activity increased by increasing the concentration of bacterial endotoxin (E. coli, 0111-B4) added to the… CONTINUE READING
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