Chromatographic resolution of lysozyme variants.

@article{FausnaughPollitt1988ChromatographicRO,
  title={Chromatographic resolution of lysozyme variants.},
  author={J Fausnaugh-Pollitt and G{\'e}raldine Th{\'e}venon and L J Janis and Fred E. Regnier},
  journal={Journal of chromatography},
  year={1988},
  volume={443},
  pages={
          221-8
        }
}
There are seven avian lysozyme variants of nearly identical three-dimensional structure which have amino acid substitutions broadly distributed on their surface. By using these protein variants, it was possible to study the relationship between protein structure and chromatographic retention. It was determined that according to the mode of separation various regions of the proteins surface determine chromatographic retention. At one extreme, immunosorbents targeted a very small region on the… CONTINUE READING

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