Chromatographic kinetic measurements of human serum albumin adsorption on monoclonal antibodies

@article{Renard1995ChromatographicKM,
  title={Chromatographic kinetic measurements of human serum albumin adsorption on monoclonal antibodies},
  author={J. Renard and C. Vidal-madjar and B. S{\'e}bille and C. Lapresle},
  journal={Journal of Molecular Recognition},
  year={1995},
  volume={8}
}
A chromatographic method was employed to study the kinetics of human serum albumin (HSA) adsorbed on immobilized monoclonal antibodies. The antibodies of various specificities were covalently bound to a high performance liquid chromatography (HPLC) silica support. For very low desorption rates, successive amounts of the reacting protein were injected until column saturation. The analysis of the increase of the non‐retained fraction calculated from peak area measurements gives the capacity of… Expand
Kinetic studies of biological interactions by affinity chromatography.
Analysis of biomolecular interactions using affinity microcolumns: a review.
  • Xiwei Zheng, Z. Li, +6 authors D. S. Hage
  • Chemistry, Medicine
  • Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
  • 2014
Analytical methods for kinetic studies of biological interactions: A review.
Establishing mathematical and physical models for the adsorption of biomacromolecules

References

SHOWING 1-10 OF 14 REFERENCES
Immunochemical cross-reactivity between cyanogen bromide fragments of human serum albumin.
SERUM ALBUMIN: AMINO ACID SEQUENCE
Kinetics of antigen-antibody reactions at solid-liquid interfaces.
...
1
2
...