Chromatographic analysis of Vinca alkaloids in human neoplastic tissues and host (mouse) tissues after injection in vivo or after incubation in vitro.

A method for extracting from biological tissues vincristine, vinblastine, and their metabolites and analysis by high-performance liquid chromatography has been developed. After excision tissues are rapidly frozen in liquid nitrogen (less than 10 s) and powders are made under liquid N2. Extraction of blood, plasma, or tissue powders was achieved using… CONTINUE READING