Chromatin structure mapping in Saccharomyces cerevisiae in vivo with DNase I.

@article{Wang2001ChromatinSM,
  title={Chromatin structure mapping in Saccharomyces cerevisiae in vivo with DNase I.},
  author={Xiande Wang and R. T. Simpson},
  journal={Nucleic acids research},
  year={2001},
  volume={29 9},
  pages={1943-50}
}
Most methods for assessment of chromatin structure involve chemical or nuclease damage to DNA followed by analysis of distribution and susceptibility of cutting sites. The agents used generally do not permeate cells, making nuclear isolation mandatory. In vivo mapping strategies might allow detection of labile constituents and/or structures that are lost when chromatin is swollen in isolated nuclei at low ionic strengths. DNase I has been the most widely used enzyme to detect chromatin sites… CONTINUE READING

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In vivo methods to analyze chromatin structure.

Current opinion in genetics & development • 1999

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