Chromatin assembly on plasmid DNA in vitro. Apparent spreading of nucleosome alignment from one region of pBR327 by histone H5.

@article{Jeong1991ChromatinAO,
  title={Chromatin assembly on plasmid DNA in vitro. Apparent spreading of nucleosome alignment from one region of pBR327 by histone H5.},
  author={She Woong Jeong and Janet Lauderdale and Arnold Stein},
  journal={Journal of molecular biology},
  year={1991},
  volume={222 4},
  pages={
          1131-47
        }
}
We have found that histone H5 (or H1) induces physiological nucleosome spacings and extensive ordering on some plasmid constructions, but not on others, in a fully defined in vitro system. Plasmid pBR327 containing DNA insertions with lengths close to 300 base-pairs permitted histone H5 to induce a remarkable degree of nucleosome alignment. Seventeen multiples of a unit 210(+/- 4) base-pair repeat, covering the entire plasmid, were detected. Plasmid pBR327, not containing a DNA insert… CONTINUE READING
BETA

Similar Papers

Loading similar papers…