Chromatin assembly in Xenopus oocytes: in vitro studies.

@article{Glikin1984ChromatinAI,
  title={Chromatin assembly in Xenopus oocytes: in vitro studies.},
  author={Gerardo C. Glikin and Ida Ruberti and Abraham Worcel},
  journal={Cell},
  year={1984},
  volume={37 1},
  pages={33-41}
}
We describe and characterize a complex reaction that catalyzes DNA supercoiling and chromatin assembly in vitro. A Xenopus oocyte extract supplemented with ATP and Mg++ converts DNA circles into minichromosomes that display a native, 200 bp periodicity. When supercoiled DNA is added to this extract it undergoes a time-dependent series of topological changes… CONTINUE READING