Chromatin Constrains the Initiation and Elongation of DNA Replication.


Eukaryotic chromosomal DNA is faithfully replicated in a complex series of cell-cycle-regulated events that are incompletely understood. Here we report the reconstitution of DNA replication free in solution with purified proteins from the budding yeast Saccharomyces cerevisiae. The system recapitulates regulated bidirectional origin activation; synthesis of… (More)
DOI: 10.1016/j.molcel.2016.10.035


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