Chorioallantoic membrane assay: vascularized 3-dimensional cell culture system for human prostate cancer cells as an animal substitute model.

@article{KunziRapp2001ChorioallantoicMA,
  title={Chorioallantoic membrane assay: vascularized 3-dimensional cell culture system for human prostate cancer cells as an animal substitute model.},
  author={Karin Kunzi-Rapp and Felicitas Genze and Rainer Kuefer and Elsa Reich and Richard E. Hautmann and J{\"u}rgen Erich Gschwend},
  journal={The Journal of urology},
  year={2001},
  volume={166 4},
  pages={1502-7}
}
PURPOSE Chorioallantoic membranes have been used as a reliable biomedical assay system for many years. Chicken eggs in the early phase of breeding are between in vitro and in vivo systems but may provide an immunodeficient, vascularized test environment. We tested this model as an in vivo system for prostate cancer research. MATERIALS AND METHODS Single cell suspensions of LNCaP, PC-3 and Tsu-Pr1 human prostatic cancer cell lines as well as 2 immortalized normal human prostate epithelial cell… CONTINUE READING