Chondroitinase ABC Digestion of the Perineuronal Net Promotes Functional Collateral Sprouting in the Cuneate Nucleus after Cervical Spinal Cord Injury

@article{Massey2006ChondroitinaseAD,
  title={Chondroitinase ABC Digestion of the Perineuronal Net Promotes Functional Collateral Sprouting in the Cuneate Nucleus after Cervical Spinal Cord Injury},
  author={James M. Massey and Charles H Hubscher and Michelle R. Wagoner and Julie A. Decker and Jeremy Amps and Jerry Silver and Stephen M. Onifer},
  journal={The Journal of Neuroscience},
  year={2006},
  volume={26},
  pages={4406 - 4414}
}
Upregulation of extracellular chondroitin sulfate proteoglycans (CSPGs) after CNS injuries contributes to the impediment of functional recovery by restricting both axonal regeneration and synaptic plasticity. In the present study, the effect of degrading CSPGs with the application of the bacterial enzyme chondroitinase ABC (chABC) into the cuneate nucleus of rats partially denervated of forepaw dorsal column axons was examined. A dorsal column transection between the C6–C7 dorsal root entry… Expand
Chondroitinase ABC promotes compensatory sprouting of the intact corticospinal tract and recovery of forelimb function following unilateral pyramidotomy in adult mice
TLDR
It is demonstrated that CSPG degradation promotes midline crossing and reinnervation of denervated target regions by intact CST axons and leads to restored function in the Denervated forepaw. Expand
Increased chondroitin sulfate proteoglycan expression in denervated brainstem targets following spinal cord injury creates a barrier to axonal regeneration overcome by chondroitinase ABC and neurotrophin-3
TLDR
Significant increases in the levels of NG2, neurocan and brevican associated with reactive glia throughout the DCN were dramatically increased despite being well beyond areas of trauma-induced blood brain barrier breakdown, demonstrating both an additional challenge and potential treatment strategy for successful functional pathway reconstruction after SCI. Expand
Chondroitinase ABC Promotes Sprouting of Intact and Injured Spinal Systems after Spinal Cord Injury
TLDR
Robust sprouting is found of both injured and intact descending projections as well as uninjured primary afferents after a cervical dorsal column injury and ChABC treatment and CSPG degradation; compensatory sprouting of descending systems could be a key mechanism underlying functional recovery. Expand
Intraspinal microinjection of chondroitinase ABC following injury promotes axonal regeneration out of a peripheral nerve graft bridge
TLDR
This study seeks to overcome the technical limitations associated with minipumps by microinjecting ChABC directly into the distal lesion site in the PN bridging model and suggests that microinjectioning Ch ABC is an effective method of delivering the potentially therapeutic enzyme directly to an injury site. Expand
Astrocytic and Vascular Remodeling in the Injured Adult Rat Spinal Cord after Chondroitinase ABC Treatment
TLDR
Interestingly, ChABC treatment favored an orientation of astrocytic processes directed toward the injury, in close association with axons at the lesion entry zone, suggesting a corr... Expand
Administration of chondroitinase ABC rostral or caudal to a spinal cord injury site promotes anatomical but not functional plasticity.
TLDR
Data suggest that ChABC-mediated sprouting of spared fibers does not necessarily translate into functional recovery, and ChABC digestion of CSPGs promotes axonal regeneration beyond a lesion site with subsequent functional improvement. Expand
IT Delivery of ChABC Modulates NG2 and Promotes GAP-43 Axonal Regrowth After Spinal Cord Injury
Chondroitin sulphate proteoglycans (CSPGs) with the major component NG2 have an inhibitory effect on regeneration of damaged axons after spinal cord injury. In this study, we investigate whether theExpand
Integrity of cortical perineuronal nets influences corticospinal tract plasticity after spinal cord injury
TLDR
The data show that changes in the expression pattern of GABAergic markers and PNNs occur in regions of the sensorimotor cortex undergoing spontaneous reorganization after SCI, but suggest that these changes have to be tightly controlled to be of functional benefit. Expand
Modulation of the proteoglycan receptor PTPσ promotes recovery after spinal cord injury
TLDR
Systemic delivery of this peptide over weeks restored substantial serotonergic innervation to the spinal cord below the level of injury and facilitated functional recovery of both locomotor and urinary systems. Expand
Chondroitin sulfate proteoglycans in spinal cord contusion injury and the effects of chondroitinase treatment.
Chondroitinase treatment of experimental spinal cord injury improves recovery of sensory, motor, and autonomic functions. Chondroitinase catalyzes the cleavage of glycosaminoglycans (GAGs) from theExpand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 69 REFERENCES
Chondroitinase ABC promotes functional recovery after spinal cord injury
TLDR
It is demonstrated that CSPGs are important inhibitory molecules in vivo and suggested that their manipulation will be useful for treatment of human spinal injuries. Expand
Axonal regeneration of Clarke’s neurons beyond the spinal cord injury scar after treatment with chondroitinase ABC
TLDR
The results show that axonal regeneration in the spinal cord can be promoted by degradation of CSPG with chondroitinase ABC, and suggests that C SPG is inhibitory to the regeneration of neurons inThe spinal cord after traumatic injury. Expand
Chondroitinase ABC promotes axonal regeneration of Clarke's neurons after spinal cord injury
TLDR
The results suggest that CS is inhibitory to the regeneration of CN neurons following injury of the spinal cord. Expand
The chondroitin sulfate proteoglycans neurocan, brevican, phosphacan, and versican are differentially regulated following spinal cord injury
TLDR
Combined glial fibrillary acidic protein (GFAP) immunohistochemistry and in situ hybridization demonstrated that GFAP astrocytes constituted a source of neurocan production after spinal cord injury, establishing a CSPG-rich matrix that persists for up to 2 months following injury. Expand
Chondroitinase ABC enhances axonal regrowth through Schwann cell‐seeded guidance channels after spinal cord injury
  • C. Chau, D. Shum, +5 authors Xm Xu
  • Chemistry, Medicine
  • FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 2004
TLDR
It is concluded that CS glycoforms deposited during gliosis at the distal graft–host interface could be cleared by the in vivo action of chondroitinase ABC to improve prospects of axonal regeneration into the host spinal cord. Expand
Synergistic Effects of Brain-Derived Neurotrophic Factor and Chondroitinase ABC on Retinal Fiber Sprouting after Denervation of the Superior Colliculus in Adult Rats
TLDR
Treatments that promote the sprouting of undamaged retinal afferents into the denervated superior colliculus after a partial retinal lesion in the adult rat are characterized and suggest that the combined treatment with BDNF and C-ABC can be relevant in therapies for the repair of the damaged adult CNS. Expand
Chondroitinase ABCI improves locomotion and bladder function following contusion injury of the rat spinal cord.
TLDR
It is demonstrated that chondroitinase is effective at promoting both somatic and autonomic motor recovery following a clinically relevant contusion spinal Cord injury and is a candidate as a therapeutic for human spinal cord injury. Expand
Degradation of Chondroitin Sulfate Proteoglycans Induces Sprouting of Intact Purkinje Axons in the Cerebellum of the Adult Rat
TLDR
The results show that, in the absence of injury or novel external stimuli, degradation of chondroitin sulfate proteoglycans is sufficient to induce Purkinje axon sprouting but not the formation of long-lasting synaptic contacts. Expand
Chronic Enhancement of the Intrinsic Growth Capacity of Sensory Neurons Combined with the Degradation of Inhibitory Proteoglycans Allows Functional Regeneration of Sensory Axons through the Dorsal Root Entry Zone in the Mammalian Spinal Cord
TLDR
Zymosan pretreatment, but not posttreatment, of the sensory ganglia, combined with ChABC modification of CSPGs resulted in robust and functional regeneration of sensory axons through the DREZ after root injury. Expand
Chondroitin sulphate proteoglycans: preventing plasticity or protecting the CNS?
TLDR
This enzyme has now been shown to restore synaptic plasticity in the visual cortex of adult rats by disrupting perineuronal nets, which contain high levels of chondroitin sulphate proteoglycans (CS‐PGs) and are expressed postnatally around groups of certain neurons in the normal CNS. Expand
...
1
2
3
4
5
...