Chondrocytes cultured in stirred suspension with serum-free medium containing pluronic-68 aggregate and proliferate while maintaining their differentiated phenotype.

@article{Gigout2009ChondrocytesCI,
  title={Chondrocytes cultured in stirred suspension with serum-free medium containing pluronic-68 aggregate and proliferate while maintaining their differentiated phenotype.},
  author={Anne Gigout and Michael D. Buschmann and Mario Jolicoeur},
  journal={Tissue engineering. Part A},
  year={2009},
  volume={15 8},
  pages={2237-48}
}
The study of chondrocyte biology requires culture conditions that maintain cell phenotype. Phenotype is rapidly lost in monolayer but is maintained in 3-dimensional scaffolds, which however, experience limited cell proliferation and limited mass transport. In this study, we cultured chondrocytes in aggregates in stirred spinner flask suspension cultures to… CONTINUE READING