Chondrocyte growth factor from the human pituitary gland.

  title={Chondrocyte growth factor from the human pituitary gland.},
  author={Susan N Kasper and I G Worsley and Jacob M. Rowe and Robert P. C. Shiu and Henry G. Friesen},
  journal={The Journal of biological chemistry},
  volume={257 9},
Extracts of frozen human pituitaries were mitogenic in a fetal rabbit chondrocyte bioassay. In the presence of 10% fetal bovine serum, a 10-fold increase in chondrocyte cell number was observed upon addition of the pituitary factor to the culture medium. After gel filtration, the chondrocyte growth factor eluted with proteins of approximately 40,000 molecular weight. These fractions were pooled and purified further upon ion exchange chromatography using DEAE-cellulose. The most active fraction… CONTINUE READING