Corpus ID: 22791705

Cholinergic stimulation of arachidonic acid and phosphatidic acid metabolism in C62B glioma cells.

  title={Cholinergic stimulation of arachidonic acid and phosphatidic acid metabolism in C62B glioma cells.},
  author={Joseph J. Degeorge and Pierre Morell and Ken D. McCarthy and Eduardo G. Lapetina},
  journal={The Journal of biological chemistry},
  volume={261 7},
Glioma C62B cells were incubated for 18 h with [1-14C]arachidonic acid. Most (80%) of the added [1-14C] arachidonic acid was taken into the intracellular pool; less than 1% of the intracellular [1-14C]arachidonic acid remained unesterified; the rest was present in glycerophospholipids. Acetylcholine stimulation of the prelabeled cells resulted in the rapid accumulation of free [1-14C]arachidonic acid, presumably liberated by hydrolysis from phospholipids. Labeled unesterified [1-14C]arachidonic… Expand
Adrenergic and cholinergic stimulation of arachidonate and phosphatidate metabolism in cultured astroglial cells
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Bradykinin effects on phospholipid metabolism and its relation to arachidonic acid turnover in neuroblastoma x glioma hybrid cells (NG 108-15).
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Bradykinin Stimulates Arachidonic Acid Release Through the Sequential Actions of an sn‐1 Diacylglycerol Lipase and a Monoacylglycerol Lipase
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In the adrenal cortex, activation of dopamine D2 receptors is coupled to a phosphoinositide-specific phospholipase-C mediated via a pertussis toxin-sensitive G-protein that inhibition of inositol phosphate and AA formation is one of the mechanisms by which dopamine controls corticosteroid production by adrenocortical cells. Expand
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It is demonstrated that unesterified arachidonate is rapidly taken up and incorporated in brain but that brain phospholipid precursor pools fail to equilibrate with plasma in short experiments, and suggests that archidonate turnover in brainospholipids is more rapid than previously assumed. Expand