Corpus ID: 22791705

Cholinergic stimulation of arachidonic acid and phosphatidic acid metabolism in C62B glioma cells.

@article{Degeorge1986CholinergicSO,
  title={Cholinergic stimulation of arachidonic acid and phosphatidic acid metabolism in C62B glioma cells.},
  author={Joseph J. Degeorge and Pierre Morell and Ken D. McCarthy and Eduardo G. Lapetina},
  journal={The Journal of biological chemistry},
  year={1986},
  volume={261 7},
  pages={
          3428-33
        }
}
Glioma C62B cells were incubated for 18 h with [1-14C]arachidonic acid. Most (80%) of the added [1-14C] arachidonic acid was taken into the intracellular pool; less than 1% of the intracellular [1-14C]arachidonic acid remained unesterified; the rest was present in glycerophospholipids. Acetylcholine stimulation of the prelabeled cells resulted in the rapid accumulation of free [1-14C]arachidonic acid, presumably liberated by hydrolysis from phospholipids. Labeled unesterified [1-14C]arachidonic… Expand
Adrenergic and cholinergic stimulation of arachidonate and phosphatidate metabolism in cultured astroglial cells
TLDR
The results suggest that cultures of astroglia can respond to several different neurotransmitters with specific changes in AA and PA metabolism, which may be of physiological significance in glial function. Expand
Bradykinin effects on phospholipid metabolism and its relation to arachidonic acid turnover in neuroblastoma x glioma hybrid cells (NG 108-15).
In neuroblastoma x glioma hybrid cells (NG 108-15) labelled with [32P]-trisodium phosphate, [3H]-inositol and [14C]-arachidonic acid, bradykinin stimulated the hydrolysis of phosphatidylinositolExpand
Bradykinin Stimulates Phosphoinositide Hydrolysis and Mobilization of Arachidonic Acid in Dorsal Root Ganglion Neurons
TLDR
The role of calcium was verified further in that accumulation of phosphatidic acid, diacylglycerol, and arachidonic acid was maximally stimulated by treatment with the calcium ionophore A23187 (20 μM) and the temporal lag in the accumulation of arachidsonic acid with respect to diglyceride andmonoglyc‐eride suggested the involvement of di‐ and monoglyceride lipases in arachIDonic acid mobilization. Expand
Phorbol ester stimulation of prostanoid synthesis by cultured astrocytes
TLDR
It is concluded that stimulation of protein kinase C by phorbol ester elicits prostanoid synthesis and release by a process that involves calcium influx and the activation of phospholipase A2. Expand
Incorporation of arachidonic and docosahexaenoic acids into phospholipids of rat brain membranes
TLDR
Results suggest that 20:4n-6 exhibits a high specific turnover in brain PI and is mobilized from this class upon relevant neuromediator stimulation, which is helpful for studying their turn over in brain. Expand
Bradykinin Stimulates Arachidonic Acid Release Through the Sequential Actions of an sn‐1 Diacylglycerol Lipase and a Monoacylglycerol Lipase
TLDR
Analysis of the molecular species profiles of individual phospholipids in DRG neurons indicated that phosphoinositide hydrolysis may account for a significant portion of the rapid increase in content of 1‐stea‐royl‐2‐arachidonoyl diacylglycerol. Expand
Stimulation of phosphoinositide hydrolysis in myelin by muscarinic agonist and potassium
TLDR
Stimulation of tissue slices with elevated K+ resulted in increased formation of inositol phosphate and decreased radioactivity in phosphatidylinositol, and all 3 phosphoinositides showed significant K+-induced loss of label, indicating they function through different mechanisms in this system. Expand
Dopamine inhibits inositol phosphate production, arachidonic acid formation, and corticosteroid release by frog adrenal gland through a pertussis toxin-sensitive G-protein.
TLDR
In the adrenal cortex, activation of dopamine D2 receptors is coupled to a phosphoinositide-specific phospholipase-C mediated via a pertussis toxin-sensitive G-protein that inhibition of inositol phosphate and AA formation is one of the mechanisms by which dopamine controls corticosteroid production by adrenocortical cells. Expand
Inositol phospholipids are probably not the source of arachidonic acid for eicosanoid synthesis in astrocytes
TLDR
Results suggest that the A23187‐stimulated release of arachidonic acid and eicosanoids is caused by aospholipase A2‐mediated attack on lipids other than the inositol phospholipids. Expand
Brain Arachidonic Acid Incorporation and Precursor Pool Specific Activity During Intravenous Infusion of Unesterified [3H]Arachidonate in the Anesthetized Rat
TLDR
It is demonstrated that unesterified arachidonate is rapidly taken up and incorporated in brain but that brain phospholipid precursor pools fail to equilibrate with plasma in short experiments, and suggests that archidonate turnover in brainospholipids is more rapid than previously assumed. Expand
...
1
2
3
4
5
...