Cholinergic and catecholaminergic receptors in the Xenopus oocyte membrane

@article{Kusano1982CholinergicAC,
  title={Cholinergic and catecholaminergic receptors in the Xenopus oocyte membrane},
  author={Kiyoshi Kusano and Ricardo Miledi and Jacques Stinnakre},
  journal={The Journal of Physiology},
  year={1982},
  volume={328}
}
1. Neurotransmitter‐receptors in the membrane of Xenopus oocytes have been studied using electrophysiological techniques. Neurotransmitters and related agents were applied while recording either membrane potential or membrane current. The majority of ovarian oocytes used were at stages IV and V. 
Pharmacological characterization of serotonin receptor induced by rat brain messenger RNA in Xenopus oocytes
TLDR
Observations indicate that serotonin actions might involve the cAMP system, as seen in applying serotonin. Expand
Recording of single γ-aminobutyrate- and acetylcholine-activated receptor channels translated by exogenous mRNA in Xenopus oocytes
TLDR
High resolution patch clamp recording in Xenopus oocytes was used to measure single channel currents from ACh- and GABA-activated receptors, which had been translated from mRNA derived from denervated cat muscle and chick optic lobe. Expand
Synthesis of chick brain GABA receptors by frog oocytes
TLDR
Poly(A)-mRNA, extracted from the optic lobe of chick embryos, directs the synthesis of γ-aminobutyric acid (GABA) receptors in Xenopus laevis oocytes, which provide a novel and useful approach to the study of brain receptors. Expand
Multiple inhibitory actions of lidocaine on Torpedo nicotinic acetylcholine receptors transplanted to Xenopus oocytes
J. Neurochem. (2011) 117, 1009–1019.
On the orientation of foreign neurotransmitter receptors in Xenopus oocytes
TLDR
It is concluded that very few, or none, of the receptor molecules expressed in the oocyte by the exogenous mRNA are inserted with the wrong orientation in the membrane. Expand
Induction of muscarinic cholinergic responsiveness inXenopus oocytes by mRNA isolated from rat brain
TLDR
The acetylcholine reaction did not require extracellular Ca ions and was suppressed by pertussis toxin, suggesting the involvement of the inhibitory guanine nucleotide-binding regulatory protein. Expand
Translation of exogenous messenger RNA coding for nicotinic acetylcholine receptors produces functional receptors in Xenopus oocytes
Messenger RNA extracted from the electric organ of Torpedo was injected into Xenopus oocytes. This led to the synthesis and incorporation of functional acetylcholine receptors into the membrane ofExpand
Messenger RNA from human brain induces drug- and voltage-operated channels in Xenopus oocytes
Sodium channels and receptors to serotonin and kainate were ‘transplanted’ from human brain into frog oocytes, by isolating messenger RNA from a fetal brain, and injecting it into Xenopus laevisExpand
Serotonin receptors induced by exogenous messenger RNA in Xenopus oocytes
TLDR
It appears that rat brain mRNA encodes the translation of serotonin receptors into the oocyte membrane, and the combination of serotonin with these receptors leads to the opening of membrane channels. Expand
Messenger RNA from insect nervous tissue induces expression of neuronal acetylcholine receptors in Xenopus oocytes.
TLDR
Immunoprecipitation experiments revealed that the coded receptor polypeptides were processed to the authentic size, and ion flux studies demonstrated that functional nicotinic acetylcholine receptors were produced and inserted into the oocyte membrane. Expand
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