Cholesterol substitution increases the structural heterogeneity of caveolae.

  title={Cholesterol substitution increases the structural heterogeneity of caveolae.},
  author={Maurice Jansen and Vilja M Pietia{\"i}nen and Harri P{\"o}l{\"o}nen and Laura Rasilainen and Mirkka Koivusalo and Ulla Ruotsalainen and Eija Jokitalo and Elina Ikonen},
  journal={The Journal of biological chemistry},
  volume={283 21},
Caveolin-1 binds cholesterol and caveola formation involves caveolin-1 oligomerization and cholesterol association. The role of cholesterol in caveolae has so far been addressed by methods that compromise membrane integrity and abolish caveolar invaginations. To study the importance of sterol specificity for the structure and function of caveolae, we replaced cholesterol in mammalian cells with its immediate precursor desmosterol by inhibiting 24-dehydrocholesterol reductase. Desmosterol could… CONTINUE READING