Cholesterol oxidase susceptibility of the red cell membrane.

  title={Cholesterol oxidase susceptibility of the red cell membrane.},
  author={Yvonne Lange and Heinrich J. G. Matthies and T L Steck},
  journal={Biochimica et biophysica acta},
  volume={769 3},
We have used the highly variable and conditional susceptibility of cholesterol oxidase to probe molecular rearrangements in the human red cell membrane. Cholesterol in the intact erythrocyte normally is not a substrate for this enzyme. Susceptibility was induced however, by these pretreatments: mild enrichment in membrane cholesterol, exposure to greater than or equal to 0.03% (3 mM) glutaraldehyde and warming in dilute salt solutions (mu approx. 0.001). Cholesterol reactivity in dilute salt… CONTINUE READING