Cholesterol modulates the interaction of beta-amyloid peptide with lipid bilayers.

@article{Qiu2009CholesterolMT,
  title={Cholesterol modulates the interaction of beta-amyloid peptide with lipid bilayers.},
  author={Liming Qiu and Anthony I. Lewis and J J Como and Mark W. Vaughn and Juyang Huang and Pentti Somerharju and Jorma A. Virtanen and Kwan Hon Cheng},
  journal={Biophysical journal},
  year={2009},
  volume={96 10},
  pages={4299-307}
}
The interaction of an amphiphilic, 40-amino acid beta-amyloid (Abeta) peptide with liposomal membranes as a function of sterol mole fraction (X(sterol)) was studied based on the fluorescence anisotropy of a site-specific membrane sterol probe, dehydroergosterol (DHE), and fluorescence resonance energy transfer (FRET) from the native Tyr-10 residue of Abeta to DHE. Without Abeta, peaks or kinks in the DHE anisotropy versus X(sterol) plot were detected at X(sterol) approximately 0.25, 0.33, and 0… CONTINUE READING