Cholesterol deprivation affects the fluorescence properties of a ceramide analog at the Golgi apparatus of living cells.

@article{Martin1993CholesterolDA,
  title={Cholesterol deprivation affects the fluorescence properties of a ceramide analog at the Golgi apparatus of living cells.},
  author={Ona C. Martin and Marcy Comly and E. Joan Blanchette-Mackie and Peter G. Pentchev and Richard E. Pagano},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1993},
  volume={90 7},
  pages={2661-5}
}
Previous studies have established that a fluorescent analog of ceramide, N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)] -6-aminohexanoyl-D-erythro-sphingosine (C6-NBD-Cer), is a vital stain for the Golgi apparatus and a useful tool for studying the sorting and transport of sphingolipids along the secretory pathway in animal cells. Here, we examine the effects of various culture conditions on labeling of the Golgi apparatus of human skin fibroblasts by C6-NBD-Cer and demonstrate that cholesterol… CONTINUE READING
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