Cholesterol-dependent macropinocytosis and endosomal escape control the transfection efficiency of lipoplexes in CHO living cells.

@article{Cardarelli2012CholesteroldependentMA,
  title={Cholesterol-dependent macropinocytosis and endosomal escape control the transfection efficiency of lipoplexes in CHO living cells.},
  author={Francesco Cardarelli and D Pozzi and Angelo Bifone and Cristina Marchini and Giulio Caracciolo},
  journal={Molecular pharmaceutics},
  year={2012},
  volume={9 2},
  pages={334-40}
}
Here we investigate the cellular uptake mechanism and final intracellular fate of two cationic liposome formulations characterized by similar physicochemical properties but very different lipid composition and efficiency for intracellular delivery of DNA. The first formulation is made of cationic lipid 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) and the zwitterionic helper dioleoylphosphocholine (DOPC), while the second one is made of the cationic 3β-[N-(N,N-dimethylaminoethane)-carbamoyl… CONTINUE READING