Cholesterol-dependent gamma-secretase activity in buoyant cholesterol-rich membrane microdomains.

  title={Cholesterol-dependent gamma-secretase activity in buoyant cholesterol-rich membrane microdomains.},
  author={Suzanne Elizabeth Wahrle and Pritam Das and Andrew C Nyborg and Chris McLendon and M Shoji and Takeshi Kawarabayashi and Linda H. Younkin and Steven G. Younkin and Todd E Golde},
  journal={Neurobiology of disease},
  volume={9 1},
Buoyant membrane fractions containing presenilin 1 (PS1), an essential component of the gamma-secretase complex, and APP CTFbeta, a gamma-secretase substrate, can be isolated from cultured cells and brain by several different fractionation procedures that are compatible with in vitro gamma-secretase assays. Analysis of these gradients for amyloid beta protein (Abeta) and CTFgamma production indicated that gamma-secretase activity is predominantly localized in these buoyant membrane microdomains… CONTINUE READING


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