Cholesterol biotransformation to androsta-1,4-diene-3,17-dione by growing cells of Chryseobacterium gleum

Abstract

Cholesterol oxidase activity was studied during biotransformation of cholesterol to androsta-1,4-diene-3,17-dione (ADD) by Chryseobacterium gleum. Spent LB media, containing cholesterol (3 mM≈1 g l−1) where the bacterium was grown for 24 h, at 30°C with constant shaking at 120 rpm, had the highest enzyme activity (167 U mg−1). The growing cells produced 0… (More)
DOI: 10.1007/s10529-010-0206-z

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