Cholera toxin and membrane gangliosides: Binding and adenylate cyclase activation in normal and transformed cells

A virally transformed, ganglioside GM1-deficient cell line binds 2% of the cholera toxin (choleragen) bound by the parent, line and is less responsive to choleragen with respect to adenylate cyclase stimulation. This biological response is maximal when 10% of choleragen-binding sites in the transformed line, or 0.5% in the parent line, are occupied. In… CONTINUE READING