Chloroplast transformation in plants: polyethylene glycol (PEG) treatment of protoplasts is an alternative to biolistic delivery systems.

@article{ONeill1993ChloroplastTI,
  title={Chloroplast transformation in plants: polyethylene glycol (PEG) treatment of protoplasts is an alternative to biolistic delivery systems.},
  author={Carmel O'Neill and G{\'a}bor V. Horv{\'a}th and Edit Horvath and Philip John Dix and P{\'e}ter Medgyesy},
  journal={The Plant journal : for cell and molecular biology},
  year={1993},
  volume={3 5},
  pages={729-38}
}
Nicotiana plumbaginifolia protoplasts were directly transformed by PEG treatment with a cloned 16S rRNA gene isolated from a double antibiotic-resistant Nicotiana tabacum plastid mutant. Putative plastid transformants were selected in cell culture by their spectinomycin resistance and identified by their unselected streptomycin resistance. Alternatively, cell lines were selected in the presence of both antibiotics. The cell line (and its regenerated plants) selected solely for spectinomycin… CONTINUE READING

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