Chloroplast replication and chloroplast DNA synthesis in spinach leaves

@article{Possingham1976ChloroplastRA,
  title={Chloroplast replication and chloroplast DNA synthesis in spinach leaves},
  author={John V. Possingham and R. J. Rose},
  journal={Proceedings of the Royal Society of London. Series B. Biological Sciences},
  year={1976},
  volume={193},
  pages={295 - 305}
}
  • J. Possingham, R. Rose
  • Published 18 May 1976
  • Environmental Science
  • Proceedings of the Royal Society of London. Series B. Biological Sciences
Cultured leaf disks from young spinach leaves have been used to study chloroplast DNA synthesis in relation to chloroplast growth and replication. Autoradiographs prepared from thin sections show that labelling throughout disks is uniform. We consider that cultured leaf disks serve as a model system for studies of cell expansion and chloroplast replication during the development of spinach leaf cells. Leaf disks from young spinach leaves were incubated continuously for 5 days in a medium… 

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References

SHOWING 1-10 OF 33 REFERENCES
Effects of Green Light on the Chloroplasts of Spinach Leaf Discs
TLDR
It is established that the synthesis of chloroplast ribosomal-RNA is similar in controls and in discs grown in low intensity green or white light, and their photosynthetic characteristics bear a similarity to those of shade plants.
Chloroplast replication and growth in tobacco
TLDR
Glucose, fructose, various vitamins, inositol, auxin, kinetin and gibberellin failed to bring the rate of chlorophyll synthesis in leaf discs on the level of that in the whole plant.
Induction and regulation of chloroplast replication in mature tobacco leaf tissue.
TLDR
Chloroplast replication was induced in mature tobacco leaf tissue by culturing leaf discs on a sterile medium composed of salts and sucrose and it appears that chloroplasts replication is related to cell expansion.
Distribution of DNA in dividing spinach chloroplasts
TLDR
In Acetabularia, which contains large numbers of chloroplasts per cell, DNA could not be detected in 65–80% of the plastids, but could easily been detected in the remainder, and in the unicellular alga, Ochromonas, 3H-thymidine is readily incorporated into chloroplast DNA.
Effect of Light Quality on Chloroplast Replication in Spinach
Chloroplast growth and replication have been studied in spinach leaf discs cultured on sterile nutrient agar under lights of different spectral qualities. Over the intensity range 0-1-5 mW cm"2 both
The localization of (3H) thymidine incorporation in the DNA of replicating spinach chloroplasts by electron-microscope autoradiography.
TLDR
It is suggested that the DNA of chloroplast is associated with the grana lamellae and extends into the stroma, and an association of chloroplast DNA molecules along the length of a continuous lamellar system would ensure an orderly segregation of DNA to daughter chloroplasts, during the binary fission of spinach chloroplastics by constriction division.
Chloroplast growth and division during the greening of spinach leaf discs.
TLDR
Effects of white and green light on chloroplast development and division during the greening of dark-grown spinach leaf discs are described.
Two Forms of Division Profile in Spinach Chloroplasts
CHLOROPLAST division is generally thought to take place by constriction (fission)1–7. Division may also occasionally occur by the growth of a central baffle before separation8–11. Usually only one
Chloroplast Replication and Growth in Tobacco
TLDR
5-Fluorodeoxyuridine inhibits chlorophyll synthesis during the normal course of "greening" by inhibiting chloroplast growth and also inhibits cytokinin-induced chloroplasts replication.
Synchronous division of plastids during the greening of cut leaves of maize.
TLDR
Results are presented showing that under precise conditions, light induces synchronous development and division of underdeveloped plastids, etioplasts, which develop into chloroplasts under illumination.
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