Chloroplast pentose‐5‐phosphate 3‐epimerase from potato: cloning, cDNA sequence, and tissue‐specific enzyme accumulation

  title={Chloroplast pentose‐5‐phosphate 3‐epimerase from potato: cloning, cDNA sequence, and tissue‐specific enzyme accumulation},
  author={Markus Teige and Stanislav Kopriva and Hermann Bauwe and K H S{\"u}ss},
  journal={FEBS Letters},
A cDNA clone encoding the chloroplast enzyme pentose‐5‐phosphate 3‐epimerase (EC in potato (Solanum tuberosum) was isolated and sequenced. The deduced sequence of 235 amino acids is similar to protein sequences of bacterial epimerases. Northern blot analysis showed the highest level of epimerase mRNA expression in potato leaves, whereas it was low in roots, tubers, and stems. Epimerase protein is mulated only in plant tissues possessing chloroplasts, i.e. in land to a lesser extent in… Expand
Molecular Cloning and Characterization of Cytosolic Isoform of Ribulose-5-Phosphate 3-Epimerase from Rice
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Structure and catalytic mechanism of the cytosolic D-ribulose-5-phosphate 3-epimerase from rice.
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Structure and mechanism of the amphibolic enzyme D-ribulose-5-phosphate 3-epimerase from potato chloroplasts.
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D-Ribulose-5-phosphate 3-epimerase: cloning and heterologous expression of the spinach gene, and purification and characterization of the recombinant enzyme.
We have achieved, to our knowledge, the first high-level heterologous expression of the gene encoding D-ribulose-5-phosphate 3-epimerase from any source, thereby permitting isolation andExpand
A Small Decrease of Plastid Transketolase Activity in Antisense Tobacco Transformants Has Dramatic Effects on Photosynthesis and Phenylpropanoid Metabolism
Transketolase (TK) catalyzes reactions in the Calvin cycle and the oxidative pentose phosphate pathway (OPPP) and produces erythrose-4-phosphate, which is a precursor for the shikimate pathwayExpand
A small decrease of plastid transketolase activity in antisense tobacco transformants has dramatic effects on photosynthesis and phenylpropanoid metabolism.
TK activity is identified as an important determinant of photosynthetic and phenylpropanoid metabolism and the provision of precursors by primary metabolism colimits flux into the shikimate pathway and phenolpropanoids metabolism are shown. Expand
The ‘high’ concentrations of enzymes within the chloroplast
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RPE, a plant gene involved in early developmental steps of nematode feeding cells
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Mining the soluble chloroplast proteome by affinity chromatography
A two‐step strategy for the enrichment of low‐abundant soluble chloroplast proteins from Pisum sativum and their subsequent identification by MS is developed, which led to the identification of 448 proteins including 43 putative novel chloroplasts. Expand


Enzymatic Evidence for a Complete Oxidative Pentose Phosphate Pathway in Chloroplasts and an Incomplete Pathway in the Cytosol of Spinach Leaves
The data indicate that chloroplasts of spinach leaf cells possess the complete complement of enzymes of the oxidative pentose phosphate path-way (OPPP), whereas the cytosol contains only the first two reactions, contrary to the widely held view that plants generally possess a cytosolic OPPP capable of cyclic function. Expand
Purification and properties of D-ribulose-5-phosphate 3-epimerase from calf liver.
  • T. Wood
  • Chemistry, Medicine
  • Biochimica et biophysica acta
  • 1979
D-Ribulose-5-phosphate 3-epimerase (EC was purified 760-fold from calf liver by adsorption on DEAE-cellulose, chromatography on DEAE-Sephadex, chromatography on D-riboseExpand
Escherichia coli rpiA gene encoding ribose phosphate isomerase A
The rpiA gene encoding ribose phosphate isomerase A was cloned from phage 1A2(471) of the Kohara gene library and data indicate that the enzyme is composed of two identical subunits. Expand
[108] d-Ribulose 5-phosphate 3-epimerase
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The Calvin cycle enzyme pentose-5-phosphate 3-epimerase is encoded within the cfx operons of the chemoautotroph Alcaligenes eutrophus
Heterologous expression of the subcloned pHG1-encoded open reading frames in Escherichia coli suggested that they were functional genes, and agreed with the finding that in A. eutrophus, PPE activity is significantly enhanced under autotrophic growth conditions which lead to a derepression of the cfx operons. Expand
Identification and characterization of the tktB gene encoding a second transketolase in Escherichia coli K-12
Results indicated that tktA and tktB were responsible for major and minor activities, respectively, of transketolase in E. coli. Expand
Plant Physiology, Biochemistry and Molecular Biology
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Domain structure of mitochondrial and chloroplast targeting peptides.
Representative samples of mitochondrial and chloroplast targeting peptides have been analyzed in terms of amino acid composition, positional amino acid preferences and amphiphilic character. NoExpand
Direct protein microsequencing from Immobilon-P Transfer Membrane.
Proteins separated by electrophoresis and electroblotted onto Immobilon-P Transfer Membrane can be sequenced directly in the gas-phase sequencer, reducing reagent use and instrument operating time. Expand