Chloroplast DNA gyrase and in vitro regulation of transcription by template topology and novobiocin

@article{Lam1987ChloroplastDG,
  title={Chloroplast DNA gyrase and in vitro regulation of transcription by template topology and novobiocin},
  author={Eric Lam and Nam-Hai Chua},
  journal={Plant Molecular Biology},
  year={1987},
  volume={8},
  pages={415-424}
}
We have examined the effects of novobiocin and template topology on the transcription of two chloroplast genes encoding the large subunit of ribulose 1,5-bisphosphate carboxylase (rbcL) and the beta subunit of the chloroplast ATPase (atpB), in an in vitro transcription system. The template topology was monitored by agarose gel electrophoresis while the in vitro transcripts were determined by 5′ S1 nuclease analysis under identical conditions. We discovered that our chloroplast transcription… CONTINUE READING

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Pea chloroplast topoisomerase I: purification, characterization, and role in replication

  • Plant Molecular Biology
  • 1988
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