Chlorophylls and Carotenoids: Measurement and Characterization by UV‐VIS Spectroscopy

@article{Lichtenthaler2001ChlorophyllsAC,
  title={Chlorophylls and Carotenoids: Measurement and Characterization by UV‐VIS Spectroscopy},
  author={H. Lichtenthaler and C. Buschmann},
  journal={Current Protocols in Food Analytical Chemistry},
  year={2001},
  volume={1}
}
The quantitative determination of chlorophyll (Chl) a, Chl b, and carotenoids in a wholepigment extract of green plant tissue by UVVIS spectroscopy is complicated by the choice of sample, solvent system, and spectrophotometer used. The various plant pigments absorb light in overlapping spectral regions, depending on the system selected. This unit discusses methods used to account for such overlap by applying equations for accurate quantitative determination of Chl a, Chl b, and total… Expand
Spectrophotometric determinations of chloroplastidic pigments in acetone, ethanol and dimethylsulphoxide
Spectrophotometric determinations of chloroplastidic pigments in acetone, ethanol and dimethylsulphoxide). The concentration of pigment indicates a range of properties in the physiological processesExpand
Plant Photosynthetic Pigments: Methods and Tricks for Correct Quantification and Identification
Chloroplast of green photosynthetic tissues in the Viridiplantae (monophyletic group that includes green algae and terrestrial plants) is characterised by a relatively conserved composition ofExpand
Reflectance spectra and images of green leaves with different tissue structure and chlorophyll content
TLDR
The results demonstrate that leaf reflectance is determined by the following basic parameters: the leaf pigment content, the leaf tissue structure, and the structure of the leaf surface. Expand
AN ADVANCED LEAF OPTICAL PROPERTIES MODEL INCLUDING PHOTOSYNTHETIC PIGMENTS
PROSPECT, a widely-used leaf directional-hemispherical reflectance and transmittance model, currently treats the behaviour of chlorophylls a, b, carotenoids, and anthocyanins uniformly, leading toExpand
Photo-Oxidative Protection of Chlorophyll a in C-Phycocyanin Aqueous Medium
TLDR
In this study, potential protection of chlorophyll a from illumination and oxidation-induced decomposition has been examined using C-phycocyanin (C-PC) aqueous medium and spectroscopy results showed that chlorine a in C-PC medium experienced the lowest rate of conversion to its derivatives; thus, it was demonstrated that chlorophyh a was mostly intact in the C- PC medium. Expand
Identification of chlorophyll pigment on Gracilaria Salicornia seaweed
Gracilaria salicornia is one of the various types of seaweed that can be commercialized because it has the potential as an antioxidant due to its high chlorophyll-a pigment. The purpose of this studyExpand
Optical spectral characterization of leaves for Quercus Resinosa and Magnolifolia species in two senescent states
Study of vegetation by non-invasive methods is a relevant topic for the preservation of the forests, by mean the biochemical analysis of vegetation, the study of the plants and analysis of theirExpand
PROSPECT-4 and 5: Advances in the leaf optical properties model separating photosynthetic pigments
Abstract The PROSPECT leaf optical model has, to date, combined the effects of photosynthetic pigments, but a finer discrimination among the key pigments is important for physiological and ecologicalExpand
Chlorophyll fluorescence imaging of photosynthetic activity in sun and shade leaves of trees
TLDR
It was shown that the photosynthetic activity of the leaves of all trees exhibits a large heterogeneity across the leaf area, and in general to a higher extent in sun leaves than in shade leaves. Expand
A methodical approach for non-destructive estimation of plant pigments by means of remission spectroscopy applied in fruit and vegetable analyses
The biological variability of plant material often leads to perturbations when optical recordings are carried out non-destructively on living horticultural products or even within the photometricExpand
...
1
2
3
4
5
...

References

SHOWING 1-3 OF 3 REFERENCES
CHLOROPHYLL AND CAROTENOIDS: PIGMENTS OF PHOTOSYNTHETIC BIOMEMBRANES
Publisher Summary This chapter presents detailed information on chlorophylls and carotenoids to give practical directions toward their quantitative isolation and determination in extracts fromExpand
Adaptation of Chloroplast-Ultrastructure and of Chlorophyll- Protein Levels to High-Light and Low-Light Growth Conditions
Abstract Adaptation In saturating light radish seedlings grown in high-light growth conditions (90 W · m-2) possess a much higher photosynthetic capacity on a chlorophyll and leaf area basis than theExpand
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.
  • D. Arnon
  • Chemistry, Medicine
  • Plant physiology
  • 1949
TLDR
Evidence that a copper enzyme, polyphenoloxidase (otherwise known as tyrosinase or catecholase), is localized in the chloroplasts of spinach beet (chard), Beta vu?garis is presented. Expand