Chlorophyll determination in intact tissues using n,n-dimethylformamide.

  title={Chlorophyll determination in intact tissues using n,n-dimethylformamide.},
  author={RichardG. Moran and D. Porath},
  journal={Plant physiology},
  volume={65 3},
Photosynthetic pigments from etiolated cucumber (Cucumis sativus var. Beit Alpha improved, Hazera Co., Gedera) cotyledons were extracted by direct immersion of the intact cotyledons into the solvent N,N-dimethylformamide (DMF). The solvent is especially efficient when pigment concentration is low; time and tools are saved and the loss of pigment that usually occurs in more complicated extraction procedures is prevented. The specific absorption coefficient of chlorophyll a in DMF was also… CONTINUE READING
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