Chitosan nanoparticles as non-viral gene delivery vehicles based on atomic force microscopy study.

@article{Yuan2009ChitosanNA,
  title={Chitosan nanoparticles as non-viral gene delivery vehicles based on atomic force microscopy study.},
  author={Yujing Yuan and J. M. A. Tan and Yifei Wang and Chui-wen Qian and Mei-ying Zhang},
  journal={Acta biochimica et biophysica Sinica},
  year={2009},
  volume={41 6},
  pages={
          515-26
        }
}
Chitosan (CS), a biocompatible and biodegradable material, can act as a non-viral delivery vehicle with low toxicity. In this study, plasmid DNA (pDNA) and siRNA were encapsulated in CS nanoparticles (NPs) to prepare CS-DNA and CS-siRNA NPs using a complex coacervation process. The CS-DNA particle size was within the range of 180-370 nm with a surface charge ranging from 0 to 18 mV at pH 5.5. The stability of pDNA in CS-DNA was investigated by pDNA release study and DNase I protection assay… 

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