Chitosan nanoparticles as non-viral gene delivery vehicles based on atomic force microscopy study.

  title={Chitosan nanoparticles as non-viral gene delivery vehicles based on atomic force microscopy study.},
  author={Yujing Yuan and J. M. A. Tan and Yifei Wang and Chui-wen Qian and Mei-ying Zhang},
  journal={Acta biochimica et biophysica Sinica},
  volume={41 6},
Chitosan (CS), a biocompatible and biodegradable material, can act as a non-viral delivery vehicle with low toxicity. In this study, plasmid DNA (pDNA) and siRNA were encapsulated in CS nanoparticles (NPs) to prepare CS-DNA and CS-siRNA NPs using a complex coacervation process. The CS-DNA particle size was within the range of 180-370 nm with a surface charge ranging from 0 to 18 mV at pH 5.5. The stability of pDNA in CS-DNA was investigated by pDNA release study and DNase I protection assay… 

Characterization and cytocompatibility of thermosensitive hydrogel embedded with chitosan nanoparticles for delivery of bone morphogenetic protein-2 plasmid DNA

The CS/CSn(pDNA-BMP2)-GP complex system exhibited excellent biological properties and cytocompatibility, indicating great potential as a gene delivery carrier and tissue regeneration scaffold for endogenous repair of the periodontium.

In vitro cytotoxicity and transfection efficiency of pDNA encoded p53 gene-loaded chitosan-sodium deoxycholate nanoparticles

The obtained results were very encouraging and offer an alternative approach to enhancing the transfection efficiency of genetic material-loaded chitosan-based delivery systems.

Chitosan/Hyaluronic Acid Nanoparticles: Rational Design Revisited for RNA Delivery.

The performance of these nonviral vectors appears difficult to predict simply on the basis of carrier- or payload-related variables, and a more holistic consideration of the journey of the nanoparticle, from cell uptake to cytosolic bioavailability of payload, is needed.

Chitosan nanoparticles as a potential nonviral gene delivery for HPV-16 E7 into mammalian cells

Chitosan nanoparticles may serve as an effective nonviral carrier for delivery of nucleotides into eukaryotic cells and transfection of CS-pEGFP NPs was efficient in CHO cells and the expression of green fluorescent proteins was well observed.


This study reviews a number of important polysaccharides with a perspective on the challenges, advantages, and disadvantages of their applications as drug/gene delivery systems.

Targeted novel surface-modified nanoparticles for interferon delivery for the treatment of hepatitis B.

The results revealed that ∼75% of the radioactivity was recovered in the liver after 4 h of injection that was nearly 3-fold greater in magnitude than the plain PLGA nanoparticles, demonstrating that the novel formulation of nanoparticles has potential application in hepatic-targeted drug delivery.

6‐O‐dodecyl‐chitosan carbamate–based pH‐responsive polymeric micelles for gene delivery

The present work reported a kind of novel pH-responsive polymeric micelles, which was derived from biopolymer of 6-O-dodecyl-chitosan carbamate (DCC) and evaluated as gene-controlled release vector and might be a promising and safe candidate as nonviral vectors for gene delivery.



Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficiency.

  • H. MaoK. Roy K. Leong
  • Biology, Chemistry
    Journal of controlled release : official journal of the Controlled Release Society
  • 2001

Transfection efficiency of chitosan vectors: effect of polymer molecular weight and degree of deacetylation.

PLGA–PEI nanoparticles for gene delivery to pulmonary epithelium

Mechanism of cell transfection with plasmid/chitosan complexes.