Chitosan nanoparticles as a potential drug delivery system for the ocular surface: toxicity, uptake mechanism and in vivo tolerance.

@article{EnrquezdeSalamanca2006ChitosanNA,
  title={Chitosan nanoparticles as a potential drug delivery system for the ocular surface: toxicity, uptake mechanism and in vivo tolerance.},
  author={Amalia Enr{\'i}quez de Salamanca and Yolanda Diebold and Margarita Calonge and Carmen Garc{\'i}a-V{\'a}zquez and Sagrario Callejo and Ana Vila and Mar{\'i}a Jos{\'e} Alonso},
  journal={Investigative ophthalmology \& visual science},
  year={2006},
  volume={47 4},
  pages={
          1416-25
        }
}
PURPOSE To study the in vitro and in vivo interaction of chitosan nanoparticles (CSNPs), a new particulate drug carrier, with epithelial cells on the ocular surface. METHODS CSNPs labeled with fluorescein isothiocyanate-bovine serum albumin were produced by ionotropic gelation. Human conjunctival epithelial cells (IOBA-NHC) were exposed for 15, 30, 60, and 120 minutes to three different CSNP concentrations. Immediately after treatment and after a 24-hour recovery period in culture medium… 
View on PubMed
ioba.med.uva.es
247 Citations
Highly Influential Citations
3
Background Citations
53
Methods Citations
10
Results Citations
8

247 Citations

Citation Type

Citation Type

Ocular drug delivery by liposome-chitosan nanoparticle complexes (LCS-NP).
Cationic gelatin nanoparticles for drug delivery to the ocular surface: in vitro and in vivo evaluation
TLDR
GPs as cationic colloidal carriers were efficiently adsorbed on the negatively charged cornea without irritating the eyes of the rabbits and can be retained in the cornea for a longer time, indicating GPs(+) have a great potential as vehicles for ocular drug delivery.
Intracellular trafficking of hyaluronic acid-chitosan oligomer-based nanoparticles in cultured human ocular surface cells
TLDR
HA-CSO NPs were internalized by two different ocular surface cell lines by an active transport mechanism, and the uptake was mediated by hyaluronan receptors through a caveolin-dependent endocytic pathway, yielding remarkable transfection efficiency.
Ocular Tolerance to a Topical Formulation of Hyaluronic Acid and Chitosan-Based Nanoparticles
TLDR
Data demonstrate that HA-CS NPs are a safe drug carrier for ocular surface application and have the potential to serve as a reliable drug delivery system to topically treat ocularsurface disorders.
Novel nanostructured lipid carrier-based inserts for controlled ocular drug delivery: evaluation of corneal bioavailability and treatment efficacy in bacterial keratitis
TLDR
NLC-based inserts developed with the addition of glycerin (Ins3OFX) was found as optimal and may be offered as appropriate vehicles for ocular delivery.
Microscopic and spectroscopic evaluation of novel PLGA-chitosan Nanoplexes as an ocular delivery system.
Intracellular Fate Investigation of Bio-Eliminable Polymeric Nanoparticles by Confocal Laser Scanning Microscopy
TLDR
Results indicate that nanoparticles were up-taken by the cells in a time dependent fashion, and preliminary evaluation of the intracellular fate of the prepared nanoparticles indicate their possible lysosomal escape.
Characterization of Chitosan-Carboxymethyl Dextran Nanoparticles as a Drug Carrier and as a Stimulator of Mouse Splenocytes
TLDR
The ability of CDNP to be the drug carrier and to enhance both humoral and cell-mediated immune response in this study demonstrated its promising potential to be applied as biomedical material.
Uptake and cytotoxicity of chitosan nanoparticles in human liver cells.
Improvement of Ocular Efficacy of Levofloxacin by Bioadhesive Chitosan Coated PLGA Nanoparticles: Box-behnken Design, In-vitro Characterization, Antibacterial Evaluation and Scintigraphy Study
TLDR
It is revealed that LFV-CS-PLGA-NPs increase the drug concentration over ocular tissue and potential usefulness for sustained drug delivery.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 37 REFERENCES
Chitosan nanoparticles: a new vehicle for the improvement of the delivery of drugs to the ocular surface. Application to cyclosporin A.
Comparative Uptake Studies of Bioadhesive and Non-Bioadhesive Nanoparticles in Human Intestinal Cell Lines and Rats: The Effect of Mucus on Particle Adsorption and Transport
TLDR
In contrast to Caco-2 cells, the presence of mucus presented a major barrier for the uptake of hydrophobic polystyrene NP and showed an even more profound effect upon the uptake at intra-duodenal administration of chitosan NP.
Study of the mechanism of interaction of poly(ϵ-caprolactone) nanocapsules with the cornea by confocal laser scanning microscopy
The potential of chitosan in ocular drug delivery
TLDR
An overview of the potential of chitosan‐based systems for improving the retention and biodistribution of drugs applied topically onto the eye and the tolerance, toxicity and biodegradation of the carriers under evaluation were reviewed.
The effect of a PEG versus a chitosan coating on the interaction of drug colloidal carriers with the ocular mucosa.
Low molecular weight chitosan nanoparticles as new carriers for nasal vaccine delivery in mice.
  • A. Vila, Alejandro Sánchez, +4 authors M. Alonso
  • Biology
    European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
  • 2004
Design of biodegradable particles for protein delivery.
A solid colloidal drug delivery system for the eye: encapsulation of pilocarpin in nanoparticles.
TLDR
The results obtained in this study show that photon correlation spectrometry is a suitable method for the sizing of nanoparticles.
Polyester Nanocapsules as New Topical Ocular Delivery Systems for Cyclosporin A
TLDR
The CyA-loaded nanocapsules are shown to be interesting vehicles for the improvement of the ocular penetration of CyA.
Design of New Formulations for Topical Ocular Administration: Polymeric Nanocapsules Containing Metipranolol
TLDR
From the in vitro release studies, it was concluded that drug diffusion through a dialysis membrane is delayed as a consequence of the encapsulation process, suggesting that drug release from these systems is governed mainly by the partition of the drug between the oily core and the aqueous release medium.
...
1
2
3
4
...