Chitosan as a carrier for non-viral gene transfer in a cystic-fibrosis cell line.

@article{Nydert2008ChitosanAA,
  title={Chitosan as a carrier for non-viral gene transfer in a cystic-fibrosis cell line.},
  author={Per Nydert and Anca Dragomir and Lena E Hjelte},
  journal={Biotechnology and applied biochemistry},
  year={2008},
  volume={51 Pt 4},
  pages={153-7}
}
The gene transfer mediated by chitosan in CFBE41o(-) (a cystic-fibrosis bronchial epithelial cell line) and HEK (a human embryonic kidney cell line) has been evaluated. Polyplexes based on chitosan and PEI (polyethyleneimine) using a luciferase and enhanced green fluorescent protein reporter plasmid showed that the transfection efficacy of polyplexes in the CFBE41o(-) cell line was poor compared with that in HEK cells. In the highly differentiated cystic-fibrosis bronchial epithelial cell line… CONTINUE READING