Chitin Induces Natural Competence in Vibrio cholerae

@article{Meibom2005ChitinIN,
  title={Chitin Induces Natural Competence in Vibrio cholerae},
  author={Karin Lederballe Meibom and Melanie Blokesch and Nadia A. Dolganov and Cheng-Yen Wu and Gary K. Schoolnik},
  journal={Science},
  year={2005},
  volume={310},
  pages={1824 - 1827}
}
The mosaic-structured Vibrio cholerae genome points to the importance of horizontal gene transfer (HGT) in the evolution of this human pathogen. We showed that V. cholerae can acquire new genetic material by natural transformation during growth on chitin, a biopolymer that is abundant in aquatic habitats (e.g., from crustacean exoskeletons), where it lives as an autochthonous microbe. Transformation competence was found to require a type IV pilus assembly complex, a putative DNA binding protein… Expand
Regulation of competence-mediated horizontal gene transfer in the natural habitat of Vibrio cholerae.
TLDR
Recent advances are presented, including the elucidation of bipartite competence regulation mediated by QstR, the inclusion of the type VI secretion system in the competence regulon of pandemic O1 El Tor strains, and the identification of TfoS as a transcriptional regulator that links chitin to competence induction in V. cholerae. Expand
Competence and natural transformation in vibrios
TLDR
Four environmental factors promote genetic competence and natural transformation in Vibrio cholerae by co‐ordinating expression of the regulators CRP, CytR, HapR and TfoX respectively. Expand
The Regulatory Network of Natural Competence and Transformation of Vibrio cholerae
TLDR
A model of the regulatory circuit of chitin-induced natural competence in V. cholerae is demonstrated and it is demonstrated that only a minority of genes involved in natural transformation are regulated in a quorum-sensing-dependent manner and that these genes determine the fate of the surrounding DNA. Expand
RpoS is required for natural transformation of Vibrio cholerae through regulation of chitinases.
  • A. B. Dalia
  • Biology, Medicine
  • Environmental microbiology
  • 2016
TLDR
It is shown that RpoS does not affect quorum sensing-dependent regulation of competence, and it is demonstrated that an rpoS mutant has reduced chit inase activity, which is required to liberate the soluble chitin oligosaccharides that serve as an inducing cue for competence. Expand
Species-specific quorum sensing represses the chitobiose utilization locus in Vibrio cholerae
TLDR
The marine facultative pathogen Vibrio cholerae forms complex multicellular communities on the chitinous shells of crustacean zooplankton in its aquatic reservoir and uses quorum sensing (QS) to regulate the expression of one locus required for V.cholerae-chitin interactions. Expand
Chitin-Induced Carbotype Conversion in Vibrio vulnificus
TLDR
It is shown that V. vulnificus can undergo chitin-dependent carbotype conversion following the uptake and recombination of complete cps loci from exogenous genomic DNA (gDNA), and that (GlcNAc)2 is the shortest chitIn-derived polymer capable of inducing competence. Expand
A transcriptional regulator linking quorum sensing and chitin induction to render Vibrio cholerae naturally transformable
TLDR
The link between chitin induction and quorum sensing is investigated and it is shown that the major regulators of these two pathways, TfoX and HapR, are both involved in the activation of a gene encoding a transcriptional regulator of the LuxR-type family, which is named QS and TFOX-dependent regulator (QstR). Expand
CytR Is a Global Positive Regulator of Competence, Type VI Secretion, and Chitinases in Vibrio cholerae
TLDR
High-throughput RNA sequencing shows that in V. cholerae, CytR is a global regulator of multiple behaviors affecting fitness and adaptability in the environment. Expand
Vibrio cholerae: Measuring Natural Transformation Frequency
TLDR
Protocols for quantifying the frequency of transformation for the human pathogen Vibrio cholerae, one of severalVibrio species recently shown to be capable of natural transformation, are described. Expand
Chitin, Cholera, and Competence
TLDR
This finding opens a new window for understanding conditions that influence the evolution of this bacterium in aquatic habitats. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 21 REFERENCES
Lysogenic Conversion by a Filamentous Phage Encoding Cholera Toxin
TLDR
The emergence of toxigenic V. cholerae involves horizontal gene transfer that may depend on in vivo gene expression, and is shown here to be encoded by a filamentous bacteriophage (designated CTXΦ), which is related to coliphage M13. Expand
Pathogenicity islands and phages in Vibrio cholerae evolution.
TLDR
To track the evolution of pathogens from their nonpathogenic progenitors, it is also crucial to identify and characterize secondary genetic elements that mediate lateral transfer of virulence genes in trans. Expand
A Glimpse into the Expanded Genome Content of Vibrio cholerae through Identification of Genes Present in Environmental Strains
TLDR
By investigating some of the nucleotide sequence basis for V. cholerae genotypic diversity, DNA fragments have been uncovered which could promote survival in coastal environments and a set of genes has been described which could be involved in as yet undiscovered interactions between V. Cholerae and eukaryotic organisms. Expand
The Vibrio cholerae chitin utilization program.
TLDR
This work shows that ChiS, a sensor histidine kinase, regulates expression of the (GlcNAc)(2-6) gene set, and identifies three sets of differentially regulated genes that confer a significant growth advantage to V. cholerae on a chitin surface. Expand
Molecular analysis of rugosity in a Vibrio cholerae O1 El Tor phase variant
TLDR
Bioinformatics analysis of expression data shows that ‘rugosity’ and ‘smoothness’ are determined by a complex hierarchy of positive and negative regulators, which also affect the biofilm, surface hydrophobicity and motility phenotypes of the organism. Expand
Parallel Quorum Sensing Systems Converge to Regulate Virulence in Vibrio cholerae
TLDR
This report identifies and characterize the genes encoding an additional V. cholerae autoinducer synthase and its cognate sensor and shows that in V.cholerae these communication systems converge to control virulence. Expand
Transcriptional Regulation of Vibrio cholerae Hemagglutinin/Protease by the Cyclic AMP Receptor Protein and RpoS
TLDR
It is demonstrated that transcription of hapA is growth phase dependent and specifically activated in the deceleration and stationary growth phases and electrophoretic mobility shift experiments showed that pure cAMP-CRP and HapR alone do not bind the haPA promoter. Expand
Quorum-sensing regulators control virulence gene expression in Vibrio cholerae
TLDR
It is shown that, in addition to the known components of the ToxR signaling circuit, quorum-sensing regulators are involved in regulation of V. cholerae virulence, and an infant mouse model found that a luxO mutant is severely defective in colonization of the small intestine. Expand
A novel CRP-dependent regulon controls expression of competence genes in Haemophilus influenzae.
TLDR
Competence-induced transcription of genes in the CRE regulon is strongly dependent on cAMP, consistent with the known role of catabolite regulation in competence, and electrophoretic mobility-shift assays confirmed that CRE sequences are a new class of CRP-binding site. Expand
Genetic transformation in freshwater: Escherichia coli is able to develop natural competence.
TLDR
The competence development of E. coli is most probably internally regulated (as for the other gram-negative bacteria), and inadequate conditions chosen for the transformation tests in the laboratory might impair the detection of higher natural transformation frequencies. Expand
...
1
2
3
...