Chiral analysis of biogenic D,L-amino acids derivatized by N-fluorenylmethoxycarbonyl-L-alanyl N-carboxyanhydride using high-performance liquid chromatography.

Abstract

Nineteen biogenic D,L-amino acids are derivatized with highly reactive N-fluorenylmethoxycarbonyl-L-alanyl N-carboxyanhydride. Using a 0.5M borate buffer at pH 7.5 and acetone, the derivatization of amino acids is completed in 5 min at room temperature. Some of the resulting diastereomeric N-protected dipeptides are successfully separated on an octylsilica stationary phase using 100mM acetate buffer (pH 4.4) and acetonitrile as the eluent.

Cite this paper

@article{Zbrozek2002ChiralAO, title={Chiral analysis of biogenic D,L-amino acids derivatized by N-fluorenylmethoxycarbonyl-L-alanyl N-carboxyanhydride using high-performance liquid chromatography.}, author={Jaroslav Zbrozek and Martin Pumera and Martin Flegel}, journal={Journal of chromatographic science}, year={2002}, volume={40 9}, pages={505-8} }