Chiral alcohol production by NADH-dependent phenylacetaldehyde reductase coupled with in situ regeneration of NADH.

  title={Chiral alcohol production by NADH-dependent phenylacetaldehyde reductase coupled with in situ regeneration of NADH.},
  author={Nobuya Itoh and Michiko Matsuda and Mariko Mabuchi and Tohru Dairi and Jincun Wang},
  journal={European journal of biochemistry},
  volume={269 9},
Phenylacetaldehyde reductase (PAR) produced by styrene-assimilating Corynebacterium strain ST-10 was used to synthesize chiral alcohols. This enzyme with a broad substrate range reduced various prochiral aromatic ketones and beta-ketoesters to yield optically active secondary alcohols with an enantiomeric purity of more than 98% enantiomeric excess (e.e.). The Escherichia coli recombinant cells which expressed the par gene could efficiently produce important pharmaceutical intermediates; (R)-2… CONTINUE READING
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Production of chiral alcohols by enantioselective reduction with NADH-dependent phenylacetaldehyde reductase from Corynebacterium strain, ST-10

  • N. Itoh, N. Mizuguchi, M. Mabuchi
  • J. Mol. Cata. B: Enzymatic
  • 1999
Highly Influential
6 Excerpts

A Pseudomonas sp. alcohol dehydrogenase with broad substrate specificity and unusual stereospecificity for organic synthesis

  • C. W. Bradshaw, H. Fu, G. J. Shen, C. H. Wong
  • J. Org. Chem
  • 1992
Highly Influential
4 Excerpts

Enzymes in Synthetic Organic Chemistry

  • Wong, C.-H, G. Whitesides
  • Pergamon, Oxford UK,
  • 1994
Highly Influential
3 Excerpts

Site-directed mutagenesis of two zinc-binding centers of the NADH-dependent phenylacetaldehyde reductase from styreneassimilating Corynebacterium sp. strain ST-10

  • Wang, J.-C, M. Sakakibara, M. Matsuda, N. Itoh
  • Biosci. Biotechnol. Biochem
  • 1999
1 Excerpt

Stereoselective oxidation and reduction by immobilized Geotrichum candidium in an organic solvent

  • K. Nakamura, Y. Inoue, T. Matsuda, I. Misawa
  • J. Chem. Soc. Perkin Trans
  • 1999

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