Chiral HPLC analysis of formoterol stereoisomers and thermodynamic study of their interconversion in aqueous pharmaceutical formulations.

  title={Chiral HPLC analysis of formoterol stereoisomers and thermodynamic study of their interconversion in aqueous pharmaceutical formulations.},
  author={Samuel O. Akapo and Chithra McCrea and June Gupta and Mark Roach and Wayne Skinner},
  journal={Journal of pharmaceutical and biomedical analysis},
  volume={49 3},

Figures and Tables from this paper


A sensitive stability indicating reverse phase high performance liquid chromatographic method was developed for the simultaneous determination of formoterol fumarate and ciclesonide in dry powder

A reverse phase HPLC method for the separation of two stereo isomers of 2-[4-(methylsulfonyl)phenyl]-3-(3(R)-oxocyclopentyl)propanoic acid.

This study describes successful method development and separation of two stereo isomers of 2-[4-(methylsulfonyl)phenyl]-3-(3(R)-oxocyclopentyl)propanoic acid by reverse phase high-performance liquid

A validated spectrophotometric method for determination of formoterol fumarate dihydrate in bulk and dosage form using methyl orange as ion pair reagent

In this study rapid, simple, accurate and sensitive spectrophotometric method has been developed for the determination of formoterol fumarate dihydrate in bulk and dosage forms. The method is based

Switchable Enantioselective Three- and Four-Dimensional Dynamic Gas Chromatography-Mass Spectrometry: Example Study of On-Column Molecular Interconversion.

The described methodology is suited to other configurationally labile molecules, which exhibit isomerization, and can be used to isolate individual compounds from multicomponent samples, without requiring pure compound synthesis, or complex mathematical models or in-silico simulations.

A simple method for the separation of (6R)- and (6S)-5,6,7,8-tetrahydrofolic acid by reversed-phase HPLC with hydroxypropyl-β-cyclodextrin as the mobile phase additive

Abstract(6R)- and (6S)-5,6,7,8-tetrahydrofolic acid were resolved by reversed-phase HPLC on a C-18 column with hydroxypropyl-β-cyclodextrin (HP-β-CD) in the mobile phase. As the concentration of

Isocratic ion pair chromatography for estimation of novel combined inhalation therapy that blocks coronavirus replication in chronic asthmatic patients

A rapid and sensitive isocratic ion-pair chromatographic method was developed for the accurate analysis of ternary mixtures of formoterol, tiotropium, and ciclesonide in their novel combined


The present work represents a UV-Spectrophotometric method for the simultaneous estimation of Formoterol Fumarate (FF) and Mometasone Furoate (MF) in respicaps dosage form. This method is based on

Determination by HPLC with electrochemical detection of formoterol RR and SS enantiomers in urine

Abstract A method is described for the determination of the R,R- and S,S-enantiomer of the long-acting s2-adrenoceptor agonist formoterol, which is marketed as a racemate for the treatment of asthma.

Isocratic HPLC Separation of Several Racemic Drugs with Two Stereogenic Centers on a Pirkle Urea-Type Chiral Stationary Phase

Abstract A simple and rapid isocratic enantiomeric separation of several racemic drugs with two stereogenic centers, namely for-moterol, labetalol, nadolol, indenolol, and U-54494A, was achieved

Stereoselective urinary excretion of formoterol and its glucuronide conjugate in human.

It is demonstrated that the urinary excretion of formoterol in male humans after oral administration of rac-formoterol is stereoselective with preferentialexcretion of the active (R; R)-formoterl as unchanged drug and glucuronide.

First High‐Performance Liquid Chromatography Assay of Formoterol Concentrations in the Low‐Picogram‐per‐Milliliter Range

The results show that this is a sensitive and reproducible method with a very low limit of detection (20 pg/ml), which creates the possibility of measuring concentrations in a range achievable in humans.

Steric aspects of agonism and antagonism at beta-adrenoceptors: synthesis of and pharmacological experiments with the enantiomers of formoterol and their diastereomers.

The enantiomers of formoterol (R;R and S;S) and their diastereomers (R;S and S;R) were synthesized and purified using a new procedure which required the preparation of the (R;R)- and (S;S)-forms of

Biological actions of formoterol isomers.

The data presented here suggest that (R,R)-formoterol binds to the beta(2) adrenoceptor and inhibits the contraction of bronchial tissues by spasmogens.

Biological significance of the enantiomeric purity of drugs.

The knowledge that enantiomers of chiral compounds may differ widely in biological activity, qualitatively as well as quantitatively, is not new. Nevertheless most of the pharmacological data

Comparison of the anti-bronchoconstrictor activities of inhaled formoterol, its (R,R)- and (S,S)-enantiomers and salmeterol in the rhesus monkey.

The data indicate that the present model of methacholine-induced bronchospasm in the rhesus monkey could be useful in defining the key properties of beta(2)agonist bronchodilators such as relative potency, efficacy, duration of action and potential for systemic side effects.

Steric aspects of formoterol and terbutaline: is there an adverse effect of the distomer on airway smooth muscle function?

There was no indication in any of the experimental approaches that (S;S)-formoterol or (S)-terbutaline might enhance the response to cholinergic or NANC-related stimuli.