Chip physically interacts with Notch and their stoichiometry is critical for Notch function in wing development and cell proliferation in Drosophila.

@article{Sachan2015ChipPI,
  title={Chip physically interacts with Notch and their stoichiometry is critical for Notch function in wing development and cell proliferation in Drosophila.},
  author={Nalani Sachan and Abhinava K. Mishra and Mousumi Mutsuddi and Ashim Mukherjee},
  journal={Biochimica et biophysica acta},
  year={2015},
  volume={1850 4},
  pages={802-12}
}
BACKGROUND Notch signaling plays a fundamental role both in metazoan cell fate determination and in the establishment of distinct developmental cell lineages. In a yeast two-hybrid screen, we identified Chip as a binding partner of Notch. Thus, we investigated the functional significance of Notch and Chip interactions. METHODS Co-immunoprecipitation and GST pull-down experiments confirmed the physical interaction between Notch and Chip. Immunostaining revealed that Chip and Notch… CONTINUE READING