Chip-based genotyping by mass spectrometry.

  title={Chip-based genotyping by mass spectrometry.},
  author={Kai Tang and Danghua Fu and D J Julien and Andreas Braun and Charles R. Cantor and Hubert K{\"o}ster},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={96 18},
Silicon chips with immobilized target DNAs were used for accurate genotyping by mass spectrometry. Genomic DNAs were amplified with PCR, and the amplified products were covalently attached to chip wells via N-succinimidyl (4-iodoacetyl)aminobenzoate (SIAB) chemistry. Primer annealing, extension, and termination were performed on a 1-microl scale directly in the chip wells in parallel. Diagnostic products thus generated were detected in situ by using matrix-assisted laser desorption ionization… CONTINUE READING
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