Chip-based dynamic real-time quantification of drug-induced cytotoxicity in human tumor cells.

@article{Wlodkowic2009ChipbasedDR,
  title={Chip-based dynamic real-time quantification of drug-induced cytotoxicity in human tumor cells.},
  author={Donald Wlodkowic and Joanna Skommer and Dagmara McGuinness and Shannon L Faley and Walter Kolch and Zbigniew Darzynkiewicz and Jonathan M. Cooper},
  journal={Analytical chemistry},
  year={2009},
  volume={81 16},
  pages={
          6952-9
        }
}
Cell cytotoxicity tests are among the most common bioassays using flow cytometry and fluorescence imaging analysis. The permeability of plasma membranes to charged fluorescent probes serves, in these assays, as a marker distinguishing live from dead cells. Since it is generally assumed that probes, such as propidium iodide (PI) or 7-amino-actinomycin D (7-AAD), are themselves cytotoxic, they are currently generally used only as the end-point markers of assays for live versus dead cells. In the… CONTINUE READING

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