Chip based biosensor for functional analysis of single ion channels

@inproceedings{Vogel2000ChipBB,
  title={Chip based biosensor for functional analysis of single ion channels},
  author={Horst Vogel},
  year={2000}
}
  • H. Vogel
  • Published 1 September 2000
  • Biology
The functional anal. of single ion channel proteins presents a serious bottleneck in the process of finding new pharmacol. active compds. Currently available single channel recording methods are not suited for automation and miniaturization. However, new techniques such as combinatorial chem. and combinatorial genetics, which produce large amts. of potential drugs and mutant proteins, demand efficient and reliable screening as well as low sample consumption. Here we present a novel, silicon… 
View via Publisher
243 Citations
Highly Influential Citations
9
Background Citations
56
Methods Citations
33
Results Citations
2

243 Citations

Natural and artificial ion channels for biosensing platforms

The single-molecule selectivity and specificity of the binding process together with the expected intrinsic gain factor obtained when utilizing flow through a channel have attracted the attention of…

Well-defined microapertures for ion channel biosensors.

This work reports on developing a stable sensory chip containing a mechanosensitive channel in a Si/SiO(2) chip with a 3 μm pore, which required only lithography and dry etching for the pore definition and membrane release and reduced the risk of membrane rupture in the fabrication process.

Micro- and nano-technologies for lipid bilayer-based ion-channel functional assays.

The current technologies that are used in the formation of ion channel-integrated bilayer lipid membranes (BLMs) in microfabricated devices as a potential platform for next-generation drug screening systems are discussed.

Generation of chip based microelectrochemical cell arrays for long-term and high-resolution recording of ionic currents through ion channel proteins

Probing the function of ionotropic and G protein-coupled receptors in surface-confined membranes.

Integrated sensor design using ion channels inserted into lipid bilayer membranes

In this paper, we present an approach towards a biosensor based on the gating action of ion channel proteins integrated on a silicon chip using planar microfabrication technology. The ion channels…

Membranes on nanopores for multiplexed single-transporter analyses

Micro- and nanostructured surfaces provide highly parallel sensor systems for the study of transmembrane processes and their use as novel biosensor platforms to elucidate non-electrogenic substrate translocation is described.

Automated formation of lipid membrane microarrays for ionic single-molecule sensing with protein nanopores.

Efficient use of membrane protein nanopores in ionic single-molecule sensing requires technology for the reliable formation of suspended molecular membranes densely arrayed in formats that allow…

Tutorial on chip-based electrophysiology to assess ion channel function

This tutorial provides a tutorial that summarizes all of the relevant work in the field of chip-based electrophysiology, especially microfabricated patch-clamp systems to date.

Reactive derivatives of gramicidin enable light- and ion-modulated ion channels

It is proposed that gA-based ion channel sensors offer tremendous potential for ultra sensitive functional detection since a single chemical modification of each individual sensing element can lead to readily detectable changes in channel conductance.
...

References

SHOWING 1-10 OF 31 REFERENCES

A biosensor that uses ion-channel switches

A biosensing technique in which the conductance of a population of molecular ion channels is switched by the recognition event, which mimics biological sensory functions and can be used with most types of receptor, including antibodies and nucleotides.

Stochastic sensing of organic analytes by a pore-forming protein containing a molecular adapter

It is shown that stochastic sensing of organic molecules can be procured from α-haemolysin by equipping the channel with an internal, non-covalently bound molecular ‘adapter’ which mediates channel blocking by the analyte.

Rapid nanopore discrimination between single polynucleotide molecules.

Because nanopores can rapidly discriminate and characterize unlabeled DNA molecules at low copy number, refinements of the experimental approach demonstrated here could eventually provide a low-cost high-throughput method of analyzing DNA polynucleotides.

Counting polymers moving through a single ion channel

This work shows that natural channel-forming peptides incorporated into a bilayer lipid membrane can be used to detect the passage of single molecules with gyration radii as small as 5–15 Å, and infer both the average number and the diffusion coefficients of poly(ethylene glycol) molecules in the pore.

Sorting single molecules: application to diagnostics and evolutionary biotechnology.

  • M. EigenR. Rigler
  • Biology, Physics
    Proceedings of the National Academy of Sciences of the United States of America
  • 1994
The method expands the horizon in molecular diagnostics by making it possible to monitor concentrations down to (less than) 10(-15) M without any need for amplification.

Supported Membranes: Scientific and Practical Applications

Supporting lipid-protein bilayers form versatile models of low-dimensionality complex fluids, which can be used to study interfacial forces and wetting phenomena, and enable the design of phantom cells to explore the interplay of lock-and-key forces and universal forces for cell adhesion.

Giant liposomes: a model system in which to obtain patch-clamp recordings of ionic channels.

It appears that the giant liposome technique offers a distinct advantage over other reconstitution procedures in that it provides a unique opportunity to undertake simultaneous biochemical, morphological, and electrophysiological studies of the incorporated ionic channel proteins.

Single binding versus single channel recordings: a new approach to study ionotropic receptors.

This work has simulated single ligand-binding events for the nicotinic acetylcholine receptor in order to provide comparisons with single channel events under pulsed agonist conditions.

Characterization of individual polynucleotide molecules using a membrane channel.

It is shown that an electric field can drive single-stranded RNA and DNA molecules through a 2.6-nm diameter ion channel in a lipid bilayer membrane, which could in principle provide direct, high-speed detection of the sequence of bases in single molecules of DNA or RNA.

A membrane fusion strategy for single‐channel recordings of membranes usually non‐accessible to patch‐clamp pipette electrodes