Chip PCR. I. Surface passivation of microfabricated silicon-glass chips for PCR.

@article{Shoffner1996ChipPI,
  title={Chip PCR. I. Surface passivation of microfabricated silicon-glass chips for PCR.},
  author={M A Shoffner and Ji-Yen Cheng and G E Hvichia and Larry J. Kricka and Peter Wilding},
  journal={Nucleic acids research},
  year={1996},
  volume={24 2},
  pages={
          375-9
        }
}
The microreaction volumes of PCR chips (a microfabricated silicon chip bonded to a piece of flat glass to form a PCR reaction chamber) create a relatively high surface to volume ratio that increases the significance of the surface chemistry in the polymerase chain reaction (PCR). We investigated several surface passivations in an attempt to identify 'PCR friendly' surfaces and used those surfaces to obtain amplifications comparable with those obtained in conventional PCR amplification systems… CONTINUE READING

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