Chimeric swine vesicular disease viruses produced by fusion PCR: a new method for epitope mapping.

@article{Dekker2000ChimericSV,
  title={Chimeric swine vesicular disease viruses produced by fusion PCR: a new method for epitope mapping.},
  author={Aldo Dekker and C. H. Leendertse and F van Poelwijk and J. M. J. Rebel and Rob J. M. Moormann},
  journal={Journal of virological methods},
  year={2000},
  volume={86 2},
  pages={131-41}
}
A new method of epitope mapping based on chimeric swine vesicular disease (SVD) viruses produced by fusion PCR (polymerase chain reaction). Seven out of 16 neutralising and non-neutralising newly produced monoclonal antibodies (MAbs) discriminated between SVD isolate ITL/1/66 and NET/1/92. Using fusion PCR eight chimeric viruses were produced containing different supplementary pieces of the P1 region of both parent strains. Using these chimeric viruses we were able to map the epitope regions… CONTINUE READING