Chimeric contribution of human extended pluripotent stem cells to monkey embryos ex vivo

  title={Chimeric contribution of human extended pluripotent stem cells to monkey embryos ex vivo
  author={Tao Tan and Jun Wu and Chenyang Si and Shaoxing Dai and Youyue Zhang and Nianqin Sun and E. Zhang and Honglian Shao and Wei Si and Pengpeng Yang and Hong Wang and Zhenzhen Chen and Ran Zhu and Yu Kang and Reyna Hern{\'a}ndez-Ben{\'i}tez and Llanos Martinez Martinez and Estrella N{\'u}{\~n}ez Delicado and William Travis Berggren and May-Fun Schwarz and Zongyong Ai and Tianqing Li and Hongkui Deng and Concepción Rodríguez Esteban and Weizhi Ji and Yuyu Niu and Juan Carlos Izpisua Belmonte},
New concepts for generating interspecies chimeras using human pluripotent stem cells
In principle, interspecies blastocyst complementation may one day allow the generation of human organs inside animals, however, some ethical issues concerning research and potential human clinical use with chimeras persist.
Reconstructing aspects of human embryogenesis with pluripotent stem cells
This stem cell platform provides insights into the design of stem cell models of embryogenesis by defining a protocol that allows for self-organizing cystic structures from human EPSCs that display some hallmarks of human early embryogenesis.
Derivation of feeder-free human extended pluripotent stem cells
Research Progress of Totipotent Stem Cells.
The totipotent stage of the early embryos, the establishment and cultivation of TSCs, and the developmental ability exploration of Tscs are summarized to promote further research of T SCs.
In Vivo Generation of Organs by Blastocyst Complementation: Advances and Challenges
This review summarizes recent advances in the field of regenerative medicine and highlights the challenges that still remain to be addressed.
Limited Expansion of Human Hepatocytes in FAH/RAG2-Deficient Swine
The presence of residual adaptive immunity in this model of immunodeficiency and the ability to induce hyposensitization in the NK cell population by taking advantage of the fetal microenvironment are confirmed.
Rethinking nomenclature for interspecies cell fusions
Advocating against describing such models as hybrids and proposing a new nomenclature for interspecies cell fusions that lacks reproductive connotations are advocated.


Domesticated cynomolgus monkey embryonic stem cells allow the generation of neonatal interspecies chimeric pigs
An optimized culture medium is reported that enhanced the anti-apoptotic ability of cmESCs and improved the development of chimeric embryos, and the capability of D-ESCs to integrate and differentiate into functional cells in a porcine model is demonstrated.
The Pluripotency Continuum and Interspecies Chimeras.
The pluripotency continuum is examined more closely in light of advances that will facilitate the production of human-animal chimeras, which is anticipated to require human chimera-competent pluripotent stem cells.
BMI1 enables interspecies chimerism with human pluripotent stem cells
It is shown that forced expression of BMI1 overcomes apoptosis of hPSCs in blastocysts of mouse, rabbit and pig allowing them to contribute to chimaeras.
Establishment of porcine and human expanded potential stem cells
The derivation of porcine EPSCs, which express key pluripotency genes, are genetically stable, permit genome editing, differentiate to derivatives of the three germ layers in chimeras and produce primordial germ cell-like cells in vitro are reported.
Transient inhibition of mTOR in human pluripotent stem cells enables robust formation of mouse-human chimeric embryos
The generation of chimera-competent naïve hPSCs unifies some common features of naïve pluripotency in mammals and may enable applications such as human organ generation in animals.