Chimeric constructs endow the human CFTR Cl- channel with the gating behavior of murine CFTR.

@article{ScottWard2007ChimericCE,
  title={Chimeric constructs endow the human CFTR Cl- channel with the gating behavior of murine CFTR.},
  author={Toby S. Scott-Ward and Zhiwei Cai and Elizabeth S Dawson and Ann O' Doherty and Ana Carina da Paula and Heather M. Davidson and David J. Porteous and Brandon J. Wainwright and Margarida D Amaral and David N Sheppard and A. C. Boyd},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2007},
  volume={104 41},
  pages={16365-70}
}
The cystic fibrosis transmembrane conductance regulator (CFTR) is a Cl(-) channel gated by ATP-driven nucleotide-binding domain (NBD) dimerization. Here we exploit species differences between human and murine CFTR to investigate CFTR channel gating. Using homologous recombination, we constructed human-murine CFTR (hmCFTR) chimeras with sequences from NBD1, NBD2, or the regulatory domain (RD) of human CFTR replaced by the equivalent regions of murine CFTR. The gating behavior of hmRD and human… CONTINUE READING