Chimeric FimH adhesin of type 1 fimbriae: a bacterial surface display system for heterologous sequences.

@article{Pallesen1995ChimericFA,
  title={Chimeric FimH adhesin of type 1 fimbriae: a bacterial surface display system for heterologous sequences.},
  author={Lars Chr Pallesen and Lars Kongsbak Poulsen and Gunna Christiansen and Paula Klemm},
  journal={Microbiology},
  year={1995},
  volume={141 ( Pt 11)},
  pages={
          2839-48
        }
}
The FimH adhesin of type 1 fimbriae has been tested as a display system for heterologous protein segments on the surface of Escherichia coli. This was carried out by introduction of restriction site handles (BglII sites) in two different positions in the fimH gene, followed by in-frame insertion of heterologous DNA segments encoding two reporter sequences. In the selected positions such insertions did not significantly alter the function of the FimH protein with regard to surface location and… CONTINUE READING

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