Chimeras of the human cytochrome P450 1A family produced in yeast. Accumulation in microsomal membranes, enzyme kinetics and stability.

@article{Bellamine1994ChimerasOT,
  title={Chimeras of the human cytochrome P450 1A family produced in yeast. Accumulation in microsomal membranes, enzyme kinetics and stability.},
  author={A Bellamine and J. C. Gautier and Philippe Urban and Denis Pompon},
  journal={European journal of biochemistry},
  year={1994},
  volume={225 3},
  pages={1005-13}
}
An expression library of hybrid cDNAs was constructed in vivo by homeologous recombination in yeast between human P450 1A1 and P450 1A2 sequences. Two clones exhibiting highly enhanced monooxygenase activities in vivo were selected. Chimera S12 includes the 88 N-terminal residues of P450 1A1 fused to the complementary part of the P450 1A2 sequence. Chimera S71 derives from P450 1A1 by the substitution of the 36 C-terminal amino acid residues by the corresponding 38 residues of the 1A2 sequence… CONTINUE READING

Citations

Publications citing this paper.

Similar Papers

Loading similar papers…