Chick embryo fibroblasts senescence in vitro: Pattern of cell division and life span as a function of cell density

@article{WeissmanShomer1975ChickEF,
  title={Chick embryo fibroblasts senescence in vitro: Pattern of cell division and life span as a function of cell density},
  author={Pnina Weissman-Shomer and Michael Fry},
  journal={Mechanisms of Ageing and Development},
  year={1975},
  volume={4},
  pages={159-166}
}
The pattern of cell division, ageing and death of cultured chick embryo fibroblasts inoculated at a wide range of cell densities is described. Cell populations seeded at densities of 2.1 x 10-3 to 3.1 x 10-4 cells per cm-2 double between 39 and 17 times respectively while their life span at all densities remains 57 puls or minus 3 days. Thus, under the experimental conditions employed, the life span of cultured embryonic chick cells is a function of calendar rather than mitotic time. We also… 
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The results show that cells serially propagated in the presence of division rate‐limiting amounts of fetal bovine serum, or at high inoculation densities, accumulate a substantial number of cells in the population during exponential growth conditions that are not senescent but are prevented from entering DNA synthesis becuase of mitogen limitations.
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    Journal of cellular physiology
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A comparison of the methods used to estimate the remaining culture lifespan indicated that the percentage of labeled nuclei was the most accurate in describing cell age.
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The lifespan of chick embryo fibroblasts maintained in primary culture for 6–73 days was examined and it was concluded that Vitamin E has no effect on the lifespan extension of chick cells.
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References

SHOWING 1-10 OF 14 REFERENCES
Doubling potential, calendar time, and donor age of human diploid cells in culture.
TLDR
It was concluded that division potential and not total calendar time was the primary determinant of the in vitro lifespan of these human diploid cells and that arresting cells from younger donors increased their ability to attain the maximum limit of their lifespan.
Cell Strain Senescence in Vitro: Cell Culture Anomaly or an Expression of a Fundamental Inability of Normal Cells to Survive and Proliferate
TLDR
This discussion is concerned mainly with the growth properties of primary cell lines, the apparently unaltered progeny of cells derived by dissociation of a given animal tissue which evolves during serial propagation of primary lines.
Enhancement of senescence in low passage human embryonic lung cells by an agent extracted from phase 3 cells.
  • G. Milo
  • Biology, Medicine
    Experimental eye research
  • 1973
TLDR
The results from parabiotic chamber experiments with solubilized membranes of aged cells, suggest that a substance released by the disruption of aged Cells enhances aging, including the removal of cells from the total population during the trypsinization process at each passage level and aging within the cell population itself.
Senescence of cultured human fibroblasts: mitotic versus metabolic time.
TLDR
The results indicate that continuously replicating cells lose viability significantly before mitotically inhibited but actively metabolizing cohorts and suggest that factors which increase cellular turnover accelerate senescence and its pathological sequelae.
THE LIMITED IN VITRO LIFETIME OF HUMAN DIPLOID CELL STRAINS.
  • L. Hayflick
  • Biology, Medicine
    Experimental cell research
  • 1965
TLDR
The survival curves obtained with human diploid cell strains are comparable to “multiple-hit” or “ multiple-target” curves obtain with other biological systems where an initial threshold dose is required before an exponential form of the curve is established.
Limited culture lifespan of human diploid cells as a function of metabolic time instead of division potential.
TLDR
Human embryonic diploid fibroblast cultures (WI-38) were maintained for periods of 3 and 5 weeks in a stationary phase by low (0.1 percent) serum medium and found limited culture lifespan may be a function of total metabolic time in vitro.
The serial cultivation of human diploid cell strains.
TLDR
A consideration of the cause of the eventual degeneration of these strains leads to the hypothesis that non-cumulative external factors are excluded and that the phenomenon is attributable to intrinsic factors which are expressed as senescence at the cellular level.
Age-related differences in intercellular adhesion for chick fibroblasts cultured in vitro.
TLDR
Combined data suggest cumulative modifications of membrane surface for in vitro ageing of chick fibroblasts through loss of intrinsic adhesiveness with ageing.
Aging in vitro: Growth of cultured cells from the Galapagos tortoise
TLDR
Skin biopsies from two growing and two fully-grown Galapagos tortoises were explanted in vitro to indicate that a proportionality exists between the potential (remaining) lifespan in vivo and the mitotic capacity of cultured diploid cells in vitro.
The division potential of cells in continuous growth as compared to cells subcultivated after maintenance in stationary phase.
Abstract Evidence is presented indicating that chick cell strains in culture have a limited survival time not directly related to the number of cell doublings undergone. It is suggested that the
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