Chestnut, American (Castanea dentata (Marsh.) Borkh.).

  title={Chestnut, American (Castanea dentata (Marsh.) Borkh.).},
  author={Charles A. Maynard and Linda D. McGuigan and Allison D. Oakes and Bo Zhang and Andrew E. Newhouse and Lilibeth C Northern and Allison M Chartrand and Logan R Will and Kathleen M. Baier and William A. Powell},
  journal={Methods in molecular biology},
The key to successful transformation of American chestnut is having the correct combination of explant tissue, selectable markers, a very robust DNA delivery system, and a reliable regeneration system. The most important components of this transformation protocol for American chestnut are the following: starting out with rapidly dividing somatic embryos, treating the embryos gently throughout the Agrobacterium inoculation and cocultivation steps, doing the cocultivation step in desiccation… CONTINUE READING