Chemotaxis of Spirochaeta aurantia: involvement of membrane potential in chemosensory signal transduction

@article{Goulbourne1981ChemotaxisOS,
  title={Chemotaxis of Spirochaeta aurantia: involvement of membrane potential in chemosensory signal transduction},
  author={E A Goulbourne and E. Peter Greenberg},
  journal={Journal of Bacteriology},
  year={1981},
  volume={148},
  pages={837 - 844}
}
The effects of valinomycin and nigericin on sugar chemotaxis in Spirochaeta aurantia were investigated by using a quantitative capillary assay, and the fluorescent cation, 3,3'-dipropyl-2,2'-thiodicarbocyanine iodide was used as a probe to study effects of chemoattractants on membrane potential. Addition of a chemoattractant, D-xylose, to cells in either potassium or sodium phosphate buffer resulted in a transient membrane depolarization. In the presence of valinomycin, the membrane potential… 
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53 Citations
Background Citations
15
Methods Citations
1
Results Citations
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53 Citations

A voltage clamp inhibits chemotaxis of Spirochaeta aurantia
TLDR
Data indicate the the action of valinomycin as a voltage clamp serves to inhibit the chemotaxis of S. aurantia and provide evidence to support the suggestion that the mechanism of Chemotaxis in this organism involves the transduction of sensory signals in the form of membrane potential fluctuations.
Involvement of transport in Rhodobacter sphaeroides chemotaxis
TLDR
Bacteria only showed chemotaxis to ammonium when grown under ammonia-limited conditions, which derepressed the ammonium transport system, and results suggest a requirement for the uptake of attractants in chemotactic sensing in R. sphaeroides.
A process related to membrane potential involved in bacterial chemotaxis to galactose.
TLDR
It is concluded that the hyperpolarization of wild-type E. coli strains is the consequence of neither galactose metabolism nor transport but rather is correlated with Galactose taxis.
Chemoattractants elicit methylation of specific polypeptides in Spirochaeta aurantia
TLDR
Chemotaxis in Spirochaeta aurantia correlates with methylation of specific polypeptides which are presumed to be analogous to the methyl-accepting chemotaxis proteins (MCPs) in bacteria such as Escherichia coli.
Mannose-Binding Lectin Inhibits the Motility of Pathogenic Salmonella by Affecting the Driving Forces of Motility and the Chemotactic Response
TLDR
An inhibitory effect of MBL is reported on the motility of pathogenic bacteria, which occurs by affecting the energy source required for motility and the signaling pathway of chemotaxis.
Enhancement of chemotaxis in Spirochaeta aurantia grown under conditions of nutrient limitation
TLDR
The results indicated that S. aurantia cells are able to regulate their chemosensory system in response to nutrient limitation, and this property provides the spirochetes with competitive advantages when the availability of nutrients becomes severely limited in their habitats.
Negative chemotaxis inSpirochaeta aurantia
A repellent-gradient tube assay for negative chemotaxis inSpirochaeta aurantia was developed and used to demonstrate that acids, alcohols, and sulfide were effective chemorepellents. The threshold…
Motility and chemotaxis of Spirochaeta aurantia: computer-assisted motion analysis
TLDR
A model to explain the behavior of S. aurantia and the response of cells to chemoattractants is described, which includes a coordinating mechanism for flagellar motor operation and a motor switch synchronizing device.
Role of proton motive force in phototactic and aerotactic responses of Rhodopseudomonas sphaeroides
TLDR
Rhodopseudomonas sphaeroides grown under nonrigorous anaerobic conditions in the light developed components of a branched respiratory electron transfer chains, and a photosynthetic electron transfer chain, and their roles in controlling tactic responses in R. sphaerides are discussed.
A model for regulation of H+-ATPase functioning in aerobical bacteria by membrane hyperpolarization caused by redox chain activation when utilizable sugars are applied
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References

SHOWING 1-10 OF 55 REFERENCES
Relationship between proton motive force and motility in Spirochaeta aurantia
TLDR
This study indicates that a proton motive force, in the form of either a transmembrane electrical potential or a trans Membrane pH gradient, is required for motility in S. aurantia.
Chemotactically induced increase in the membrane potential of spheroplasts of Rhodopseudomonas sphaeroides
Change in intracellular pH of Escherichia coli mediates the chemotactic response to certain attractants and repellents
TLDR
The pathway of sensory transduction which proceeds through methyl-accepting chemotaxis protein I was found to be involved in the response to a change in intracellular pH.
Sensory electrophysiology of bacteria: relationship of the membrane potential to motility and chemotaxis in Bacillus subtilis.
  • J. B. MillerD. Koshland
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1977
TLDR
Change of the overall membrane potential of a normal B. subtilis is not required for chemotaxis, but such a change is sensed by the bacteria just as changing levels of attractants and repellents are sensed.
Chemotaxis in Spirochaeta aurantia
TLDR
Tgrowing cells of S. aurantia M1 exhibited an aerotactic response, and d-Galactose taxis and D-fucose taxis were induced by the presence of D-galactose in the growth medium.
Change in membrane potential during bacterial chemotaxis.
  • S. SzmelcmanJ. Adler
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1976
To find out if there are changes in membrane potential during bacterial chemotaxis, we measured the membrane potential of Escherichia coli indirectly by use of the permeating, lipid-soluble cation…
Protonmotive force and motility of Bacillus subtilis
TLDR
Motility of Bacillus subtilis was inhibited within a few minutes by a combination of valinomycin and a high concentration of potassium ions in the medium at neutral pH, which is easily explained by the idea that the motility of B. subtILis is supported by the electrochemical potential difference of the proton across the membrane, or theProtonmotive force.
Source of Energy for Gliding Motility in Flexibacter polymorphus: Effects of Metabolic and Respiratory Inhibitors on Gliding Movement
  • H. Ridgway
  • Biology, Chemistry
    Journal of bacteriology
  • 1977
TLDR
It is suggested that ATP may not function as the sole energy donor for the gliding mechanism, but that some additional product of electron transport is required (e.g., the intermediate of oxidative phosphorylation).
Involvement of cyclic GMP in intracellular signaling in the chemotactic response of Escherichia coli.
TLDR
It is suggested that cyclic GMP (cGMP) is involved in this signaling process in chemotaxis by Escherichia coli, and mutants defective in components of the adaptation system have altered cGMP metabolism.
Cytoplasmic pH mediates pH taxis and weak-acid repellent taxis of bacteria
TLDR
It is concluded that taxes away from acid pH and membrane-permeant weak acids are both mediated by a pH-sensitive component located either in the cytoplasm or on the cy toplasmic side of the membrane, rather than by an external receptor (as in the case of the attractants).
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