Chemostat-based proteomic analysis of toluene-affected Pseudomonas putida S12.

@article{Volkers2006ChemostatbasedPA,
  title={Chemostat-based proteomic analysis of toluene-affected Pseudomonas putida S12.},
  author={Rita J. M. Volkers and Ad L de Jong and Albert G. Hulst and Ben M L van Baar and Jan A. M. de Bont and Jan Wery},
  journal={Environmental microbiology},
  year={2006},
  volume={8 9},
  pages={
          1674-9
        }
}
The aim of this study was to assess the cellular response of the solvent-tolerant Pseudomonas putida S12 to toluene as the single effector. Proteomic analysis (two-dimensional difference-in-gel-electrophoresis) was used to assess the response of P. putida S12 cultured in chemostats. This approach ensures constant growth conditions, both in the presence and absence of toluene. A considerable negative effect of toluene on the cell yield was found. The need for energy in the defence against… CONTINUE READING
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